ISOLATION AND CHARACTERIZATION OF LIPASE-PRODUCING FUNGI FROM SOUTHERN COASTAL WATERS OF THE CASPIAN SEA BY ITS-PCR

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SADATI R.; NAZEMI A.; BARGHI A.

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Journal: Biotechnological Journal of Environmental Microorganisms Year:2012 | Volume: | Issue: Page(s): 33-43

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Title

ISOLATION AND CHARACTERIZATION OF LIPASE-PRODUCING FUNGI FROM SOUTHERN COASTAL WATERS OF THE CASPIAN SEA BY ITS-PCR

Author(s)

SADATI R. | NAZEMI A. | BARGHI A. | Issue Writer Certificate

Keywords

MARINE FUNGIQ2

LIPASEQ2

CASPIAN SEA COASTQ2

ITS-PCRQ2

Abstract

Caspian Sea is one of the most valuable ecosystems in the world and contain a high diversity of fungal species with capable of producing the enzyme. LIPASEs (triacylglycerol acyl hydrolases, EC 3.1.1.3) catalyze the hydrolysis and the synthesis of esters formed from glycerol and long-chain fatty acids. LIPASEs occur widely in nature, but only microbial LIPASEs are commercially significant.Microbial LIPASEs are currently receiving much attention with the rapid development of enzyme technology. The aim of this study was isolation and identification of LIPASE-producing fungi from the coastal waters of the Caspian Sea. In this study, 25 fungal species were isolated from southern coasts of the Caspian Sea in spring 2011 and tested to determine their lipolytic activity by screening techniques on tributyrin agar media. Four LIPASE-producing fungi were selected and their PCR identification and sequencing were carried out. Determination of the nucleotide sequencing of the ITS-5.8S rDNA gene showed that these isolated species belonged to genera Cladosporium, Glomerella and Phaeosphaeria. Among them, two species of Glomerella acutata and Cladosporium cladosporioides showed higher LIPASE activity.

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APA: Copy

SADATI, R., NAZEMI, A., & BARGHI, A.. (2012). ISOLATION AND CHARACTERIZATION OF LIPASE-PRODUCING FUNGI FROM SOUTHERN COASTAL WATERS OF THE CASPIAN SEA BY ITS-PCR. BIOTECHNOLOGICAL JOURNAL OF ENVIRONMENTAL MICROORGANISMS, 6(3 (3)), 33-43. SID. https://sid.ir/paper/242225/en

Vancouver: Copy

SADATI R., NAZEMI A., BARGHI A.. ISOLATION AND CHARACTERIZATION OF LIPASE-PRODUCING FUNGI FROM SOUTHERN COASTAL WATERS OF THE CASPIAN SEA BY ITS-PCR. BIOTECHNOLOGICAL JOURNAL OF ENVIRONMENTAL MICROORGANISMS[Internet]. 2012;6(3 (3)):33-43. Available from: https://sid.ir/paper/242225/en

IEEE: Copy

R. SADATI, A. NAZEMI, and A. BARGHI, “ISOLATION AND CHARACTERIZATION OF LIPASE-PRODUCING FUNGI FROM SOUTHERN COASTAL WATERS OF THE CASPIAN SEA BY ITS-PCR,” BIOTECHNOLOGICAL JOURNAL OF ENVIRONMENTAL MICROORGANISMS, vol. 6, no. 3 (3), pp. 33–43, 2012, [Online]. Available: https://sid.ir/paper/242225/en

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