A comparison of egg yolk, soybean lecithin, and bovine serum albumin on bull semen fertility and cryopreservability

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Taghavi Chamazkoti A.; MOSLEMIPUR F.; MOSTAFALOO Y.; GHANBARI F.

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Journal: JOURNAL OF RUMINANT RESEARCH Year:2018 | Volume:6 | Issue:1 Page(s): 147-154

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Title

A comparison of egg yolk, soybean lecithin, and bovine serum albumin on bull semen fertility and cryopreservability

Author(s)

Taghavi Chamazkoti A. | MOSLEMIPUR F. | MOSTAFALOO Y. | GHANBARI F. | Issue Writer Certificate

Keywords

Semen extenderQ1

Sperm fertilityQ1

Bovine serum albuminQ1

CowQ3

Abstract

Background and objectives: The storing and cryopreservation of livestock semen for a long time enables the use of better genetic reserves. Damages from peroxidation and cold shock are indispensable part of long time storage which leads to a lowered semen fertility. So, it is needed to provide greater insight into the effects of different materials in Semen extender to improve fertility. In this study, three experiments were implemented to investigate the effect of different soybean lecithin instead of egg yolk in Tris extender and also adding Bovine serum albumin on fertility traits of bovine semen stored under three thermal conditions; room temperature (24° C), chilling temperature (5° C), and frozen in liquid nitrogen. Materials and methods: Semen samples were collected from four Holstein bulls (about three years old) using artificial vagina and pooled after initial quality evaluation. Semen samples then were extended by four different extenders containing egg yolk (20%) and three levels of soy lecithin (0. 5, 1 and 1. 5%). Before filling 0. 5 ml straws with extended semen, 10 mg Bovine serum albumin was added to the half of straws. Straws were stored at three thermal conditions; under room temperature and evaluation after 3, 6 and 9 hrs under chilling temperature and evaluation after 3, 24, and 48 hrs under freezing condition and then evaluation of thawed straws at days 5, 10 and 15 post-freezing. Sperm concentrations, viability and motility were evaluated microscopically. Data were analyzed as a 2 × 4 × 3 factorial experiment (including extender, Bovine serum albumin and time effects) with a completely randomized design. Means comparison was by the least significant difference test. Results: Results from the experiment under room temperature showed that the effects of extender and time on sperm viability and motility were significant (p<0. 01) but adding albumin had no significant effect on these parameters. The highest sperm viability and motility percentages were 99. 88 and 99. 75 in egg yolk group at 3 hrs after storage, respectively. Regarding the results from the chilling condition, the effects of extender and time on sperm viability and motility were significant (p<0. 01) but adding albumin showed no significant effect. The highest sperm viability and motility percentages were 98. 34 and 97. 67 in egg yolk group at 3 hrs after storage, respectively. The effects of extender and time on sperm viability and the effect of extender, albumin and time on sperm motility after thawing were significant (p<0. 05). The highest sperm viability and motility percentages were 68. 18 and 65. 62 in egg yolk group at day 5 post-freezing, respectively. Conclusion: Generally, results of the study showed that using egg yolk in Tris Semen extender in comparison to soybean lecithin has priority for storing bull semen in liquid and frozen conditions. Furthermore, adding Bovine serum albumin into extender was not effective on bovine sperm viability and motility.

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APA: Copy

Taghavi Chamazkoti, A., MOSLEMIPUR, F., MOSTAFALOO, Y., & GHANBARI, F.. (2018). A comparison of egg yolk, soybean lecithin, and bovine serum albumin on bull semen fertility and cryopreservability. JOURNAL OF RUMINANT RESEARCH, 6(1 ), 147-154. SID. https://sid.ir/paper/244084/en

Vancouver: Copy

Taghavi Chamazkoti A., MOSLEMIPUR F., MOSTAFALOO Y., GHANBARI F.. A comparison of egg yolk, soybean lecithin, and bovine serum albumin on bull semen fertility and cryopreservability. JOURNAL OF RUMINANT RESEARCH[Internet]. 2018;6(1 ):147-154. Available from: https://sid.ir/paper/244084/en

IEEE: Copy

A. Taghavi Chamazkoti, F. MOSLEMIPUR, Y. MOSTAFALOO, and F. GHANBARI, “A comparison of egg yolk, soybean lecithin, and bovine serum albumin on bull semen fertility and cryopreservability,” JOURNAL OF RUMINANT RESEARCH, vol. 6, no. 1 , pp. 147–154, 2018, [Online]. Available: https://sid.ir/paper/244084/en

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