During 1998-99 growing season the present study was conducted to isolate and identify Fusarium species from root and crown of wheat and barley in Khuzestan province. So, infected plants were collected from different locations including: Andimeshk, Ahwaz, Izeh, Hamidieh, Khoramshahr, Dezful, Susangerd, Shadegan, Shavour, Shush, Shushtar, Abd-el-Khan, Mahshahr, Mollasani, Hafttape, and Hoveizeh. Fungal isolation was made by plating surface sterilized segments of rotted root and crown of plants on PDA and Nash & Snyder media. One hundred and three isolates of Fusarium were isolated. Among fungal isolates, 12 species were identified as follows: F. semitectum, F. solani, F. proliferatum, F. subglutinans, F. oxysporum, F. moniliforme, F. equisei, F. longipes, F. lateritium, F. sambucinum, F. diversisporum and F. anthophilum. Among species, F.semitectum was the most frequent (23.3%) and isolated from both plants in the most areas, and F. anthophilum was the least frequent (0.97%) and isolated from wheat only in Shadegan. It seems that, this is the first repot of occurrence of F. longipes and F.diversisporum on roots-and crowns of wheat and barley and F. anthophilum on the wheat crown in the world. Also, occurrence of F. lateritium, F. solani and F. subglutinans on the barley is new for Iran.