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Information Journal Paper

Title

THE EFFECT OF DIFFERENT HORMONES ON CALLUS INDUCTION, REGENERATION AND MULTIPLICATION OF SAFFRON (CROCUS SATIVUS L.) CORMS

Pages

  143-154

Abstract

 Saffron (Crocus sativus; Iridaceae) is an important economic and medicinal crop in Iran. The saffron that is an herbaceous triploid geophyte is used mainly as a source of secondary metabolites having aromatic and medicinal value. The plant develops annually from buds on the mother corm- i.e. a thickened stem- which acts as a resting, perennating storage organ. In this researchin vitro propagation ofCrocus sativus through direct and indirect methods has been studied. Corm slices as explants were sterilized and transferred to MS media with different concentrations of plant GROWTH REGULATORS. The results showed that use of HgCl2 0.15% for 20 minutes is the best treatment for sterilization. The results of ANOVA of direct regeneration indicated that the most corm formation was observed in 2 mg/l BAP. Maximum callus induction was achieved on MS supplemented with 1 mg/l 2,4-D+2 mg/l BAP. As results indicate the most number of EMBRYOs were obtained in MS medium containing 0.15 mg/ l NAA. Also this medium was suitable for germination of EMBRYO. Corm formation was only observed in MS supplemented with1 mg/l 2,4-D+2 mg/l BAP in the indirect method. The corm was transferred to JF pot for adaptation and then it was transferred to the pot and soil for growing.

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    APA: Copy

    SAFARNEJAD, ABBAS, ALAMDARI, SEYEDEH BIBI LEYLA, DARROUDI, HADI, & DALIR, MARZIEH. (2016). THE EFFECT OF DIFFERENT HORMONES ON CALLUS INDUCTION, REGENERATION AND MULTIPLICATION OF SAFFRON (CROCUS SATIVUS L.) CORMS. SAFFRON AGRONOMY AND TECHNOLOGY, 4(2), 143-154. SID. https://sid.ir/paper/247941/en

    Vancouver: Copy

    SAFARNEJAD ABBAS, ALAMDARI SEYEDEH BIBI LEYLA, DARROUDI HADI, DALIR MARZIEH. THE EFFECT OF DIFFERENT HORMONES ON CALLUS INDUCTION, REGENERATION AND MULTIPLICATION OF SAFFRON (CROCUS SATIVUS L.) CORMS. SAFFRON AGRONOMY AND TECHNOLOGY[Internet]. 2016;4(2):143-154. Available from: https://sid.ir/paper/247941/en

    IEEE: Copy

    ABBAS SAFARNEJAD, SEYEDEH BIBI LEYLA ALAMDARI, HADI DARROUDI, and MARZIEH DALIR, “THE EFFECT OF DIFFERENT HORMONES ON CALLUS INDUCTION, REGENERATION AND MULTIPLICATION OF SAFFRON (CROCUS SATIVUS L.) CORMS,” SAFFRON AGRONOMY AND TECHNOLOGY, vol. 4, no. 2, pp. 143–154, 2016, [Online]. Available: https://sid.ir/paper/247941/en

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