INCREASE OF P5CS GENE EXPRESSION IN OLIVE PLANTLETS UNDER SALINITY STRESS

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FARZANEH BEHELGARDI M.; MOTAMED N.; RASTGAR JAZI F.; EBRAHIMZADEH H.

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Journal: Journal of Molecular and Cellular Researches Year:2014 | Volume:27 | Issue:1 Page(s): 119-124

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Title

INCREASE OF P5CS GENE EXPRESSION IN OLIVE PLANTLETS UNDER SALINITY STRESS

Author(s)

FARZANEH BEHELGARDI M. | MOTAMED N. | RASTGAR JAZI F. | EBRAHIMZADEH H. | Issue Writer Certificate

Keywords

OLIVEQ2

P5CSQ2

OSMOTIC STRESSQ2

POLY CLONAL ANTIBODYQ2

Abstract

Salinity and drought are the most important environmental factors causing OSMOTIC STRESS and limit intensely plant growth. Production of osmolyts such as proline is a current response in many plants under osmotic tension. The key enzyme of proline biosynthesis pathway is P5CS (D1-pyrroline- 5-carboxylate synthetase). In this study, embryos of the OLIVE cv. Zard were cultured on solid 1/2 MS media, after 4 weeks, half of the plantlets were transferred to the solid 1/2 MS containing 200 mM NaCl and they were sub- cultured about 2 weeks, then total protein was extracted from fresh leaves of both plantlets under stress condition and non stress condition and measured using of brad ford to subject to SDS-PAGE gel electrophoresis. To probe the effect of P5CS overexpression on salinity stress tolerance, POLY CLONAL ANTIBODY developed in rabbit against P5CS protein was used. The result of immunological methods for specific detection of P5CS gene, strongly indicated P5CS up-regulation in stressed plantlets versus non stressed plantlets and these results implicate the key role of proline in salinity stress toleration.

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APA: Copy

FARZANEH BEHELGARDI, M., MOTAMED, N., RASTGAR JAZI, F., & EBRAHIMZADEH, H.. (2014). INCREASE OF P5CS GENE EXPRESSION IN OLIVE PLANTLETS UNDER SALINITY STRESS. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 27(1), 119-124. SID. https://sid.ir/paper/248441/en

Vancouver: Copy

FARZANEH BEHELGARDI M., MOTAMED N., RASTGAR JAZI F., EBRAHIMZADEH H.. INCREASE OF P5CS GENE EXPRESSION IN OLIVE PLANTLETS UNDER SALINITY STRESS. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2014;27(1):119-124. Available from: https://sid.ir/paper/248441/en

IEEE: Copy

M. FARZANEH BEHELGARDI, N. MOTAMED, F. RASTGAR JAZI, and H. EBRAHIMZADEH, “INCREASE OF P5CS GENE EXPRESSION IN OLIVE PLANTLETS UNDER SALINITY STRESS,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 27, no. 1, pp. 119–124, 2014, [Online]. Available: https://sid.ir/paper/248441/en

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