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Information Journal Paper

Title

GENETIC DIVERSITY OF MALUS ORIENTALIS IN HYRCANIAN FOREST USING ISSR-PCR MARKERS

Pages

  359-369

Abstract

 Apple (Malus orientalis.) distributed throughout the HYRCANIAN FOREST from lowland regions to steep and mountainous areas. For evaluation GENETIC DIVERSITY, leaf material were collected from 104 individual of were 14 population. DNA was extracted and DNA polymorphism were considered using four primers (GA)8YC, (AC)8YA,(AG)8AT and (AC)8G with ISSR-PCR method. The results showed that for four primers was detected 385 allele with 0.049 heterozygosiyt.The mean heterozygosity was 0.049 and varied from 0.031 in “Afratakhte” population to 0.059 in “kodir” and “Lamzer” populations. The maximum Nei genetic distance was belong to “Siahbill” and “Masal” populations and the minimum was related to “Yoush”, “Afratakhte” and “Dinekooh”. the AMOVA result indicated that the intra and inter population diversity were 94% and 6%, respectively that indicate significant within population diversity of this species.Despite the different habitat conditions and long geographical distance among populations, The low genetic differentiation (excluding Siahbill population) and similar heterozygosity within populations suggest high gene flow among populations of Malus orientalis in north of Iran.

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  • Cite

    APA: Copy

    KHODADOST, A., YOUSEFZADEH, H., AMIRCHAKHMAGHI, N., ABDOLLAHI, H., & HOSSEINZADEH COLAGAR, A.. (2016). GENETIC DIVERSITY OF MALUS ORIENTALIS IN HYRCANIAN FOREST USING ISSR-PCR MARKERS. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 29(4 ), 359-369. SID. https://sid.ir/paper/248547/en

    Vancouver: Copy

    KHODADOST A., YOUSEFZADEH H., AMIRCHAKHMAGHI N., ABDOLLAHI H., HOSSEINZADEH COLAGAR A.. GENETIC DIVERSITY OF MALUS ORIENTALIS IN HYRCANIAN FOREST USING ISSR-PCR MARKERS. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2016;29(4 ):359-369. Available from: https://sid.ir/paper/248547/en

    IEEE: Copy

    A. KHODADOST, H. YOUSEFZADEH, N. AMIRCHAKHMAGHI, H. ABDOLLAHI, and A. HOSSEINZADEH COLAGAR, “GENETIC DIVERSITY OF MALUS ORIENTALIS IN HYRCANIAN FOREST USING ISSR-PCR MARKERS,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 29, no. 4 , pp. 359–369, 2016, [Online]. Available: https://sid.ir/paper/248547/en

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