TOXICITY ASSAY OF HEAVY METALS PB, CD AND CU BY LUMINESCENT BACTERIUM ISOLATED FROM THE CASPIAN SEA

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MOHSENI M.; MAGHOOL SH.S.

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Journal: Journal of Molecular and Cellular Researches Year:2015 | Volume:28 | Issue:4 Page(s): 588-598

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Title

TOXICITY ASSAY OF HEAVY METALS PB, CD AND CU BY LUMINESCENT BACTERIUM ISOLATED FROM THE CASPIAN SEA

Author(s)

MOHSENI M. | MAGHOOL SH.S. | Issue Writer Certificate

Keywords

LUMINESCENT BACTERIUMQ2

HEAVY METALSQ2

TOXICITY ASSAYQ2

CASPIAN SEAQ2

Abstract

Due to the widespread of environmental pollutants and HEAVY METALS, the use of modern, fast and high performance methods for detection of the pollutants has great significance. One of the methods to assay the toxicity of environmental samples is the use of luminescent bacteria for the assay based on bioluminescence inhibition. The purpose of the current study was TOXICITY ASSAY of HEAVY METALS using LUMINESCENT BACTERIUM isolated from the CASPIAN SEA. Water samples were collected from different depths of the CASPIAN SEA. For the isolation of luminescent bacteria, samples were inoculated into specific medium and incubated at 28 °C. Growth and luminescence of the isolated were studied using spectrophotometry and luminometry. Then the assessment of the HEAVY METALS toxicity including Pb, Cd and Cu was performed using the luminescence property of the isolate. A LUMINESCENT BACTERIUM was isolated from sea water and named MM1. The result of bioluminescence investigation revealed that the isolate MM1 can be able to emit a steady and high level of light. The light emission of isolate MM1 was significantly decreased in the presence of HEAVY METALS. The results revealed that the isolate was able to detect low concentration of HEAVY METALS. The EC50 values for Pb, Cd and Cu were assayed 8.55, 18.46 and 3.77 mgL-1, respectively. The 16S rDNA sequencing was exhibited that the MM1 strain was similar toVibrio harveyi with 99% homology. The results of this study demonstrated that the luminescence activity of bacterium isolated from the CASPIAN SEA was sensitive to the presence of HEAVY METALS and was significantly reduced. The isolate can be used for TOXICITY ASSAY of environmental pollutants especially for HEAVY METALS.

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APA: Copy

MOHSENI, M., & MAGHOOL, SH.S.. (2015). TOXICITY ASSAY OF HEAVY METALS PB, CD AND CU BY LUMINESCENT BACTERIUM ISOLATED FROM THE CASPIAN SEA. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 28(4), 588-598. SID. https://sid.ir/paper/248576/en

Vancouver: Copy

MOHSENI M., MAGHOOL SH.S.. TOXICITY ASSAY OF HEAVY METALS PB, CD AND CU BY LUMINESCENT BACTERIUM ISOLATED FROM THE CASPIAN SEA. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2015;28(4):588-598. Available from: https://sid.ir/paper/248576/en

IEEE: Copy

M. MOHSENI, and SH.S. MAGHOOL, “TOXICITY ASSAY OF HEAVY METALS PB, CD AND CU BY LUMINESCENT BACTERIUM ISOLATED FROM THE CASPIAN SEA,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 28, no. 4, pp. 588–598, 2015, [Online]. Available: https://sid.ir/paper/248576/en

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