THE EFFECT OF VITRIFICATION ON PLURIPOTENCY SPECIFIC GENES, OCT4 AND NANOG IN MOUSE BLASTOCYSTS

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FATHALIZADEH P.; DASHTIZAD M.; ASAADI TEHRANI G.; DALIRI JOUPARI M.

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Journal: Journal of Molecular and Cellular Researches Year:2015 | Volume:28 | Issue:4 Page(s): 568-578

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Title

THE EFFECT OF VITRIFICATION ON PLURIPOTENCY SPECIFIC GENES, OCT4 AND NANOG IN MOUSE BLASTOCYSTS

Author(s)

FATHALIZADEH P. | DASHTIZAD M. | ASAADI TEHRANI G. | DALIRI JOUPARI M. | Issue Writer Certificate

Keywords

VITRIFICATIONQ2

MOUSE BLASTOCYSTQ1

OCT4 AND NANOG GENESQ2

Abstract

From the first VITRIFICATION ofmouse embryos in 1985, considerable efforts have been made to develop and improve cryopreservation techniques, but still biochemical and fetal chromosomal abnormalities caused by VITRIFICATION can cause changes in the pattern of genes expression involved in embryonic development and function, reduced implantation and fetal death. The purpose of this study was to investigate the effects of VITRIFICATION on survival and the expression of pluripotency specific genes likeOct4 and Nanogin the vitrified-warmed MOUSE BLASTOCYSTs. Harvested blastocysts from superovulatedfemale mice were vitrified in the presence of dimethyl sulfoxide 15%, ethylene glycol 15% and sucrose 0.5 M as cry oprotectants and vitrified in liquid nitrogen after loading on Cryotop. Thawing process was performed by immersing the blastocysts in thawing solution prepared using sucrose 1 M. Survival and hatching rates were evaluated and also theOct4 and Nanog genes expression in vitrified- thawed and fresh blastocysts (control group) were assessed by real time-PCR method. Survival and hatching rates decreased significantly in vitrified blastocysts compared with control group (94.43 ± 5.81% vs.100% for survival and 57.78 ± 11.76% vs.86.88 ± 5.80% for hatching). Also increased expression of Nanog and Oct4 genes were observed in vitrified- warmed blastocysts. Considering the results of this study, it can be said that VITRIFICATION ofmouse embryo at the blastocyst stage can change gene expression and quality of the embryo.

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APA: Copy

FATHALIZADEH, P., DASHTIZAD, M., ASAADI TEHRANI, G., & DALIRI JOUPARI, M.. (2015). THE EFFECT OF VITRIFICATION ON PLURIPOTENCY SPECIFIC GENES, OCT4 AND NANOG IN MOUSE BLASTOCYSTS. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 28(4), 568-578. SID. https://sid.ir/paper/248578/en

Vancouver: Copy

FATHALIZADEH P., DASHTIZAD M., ASAADI TEHRANI G., DALIRI JOUPARI M.. THE EFFECT OF VITRIFICATION ON PLURIPOTENCY SPECIFIC GENES, OCT4 AND NANOG IN MOUSE BLASTOCYSTS. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2015;28(4):568-578. Available from: https://sid.ir/paper/248578/en

IEEE: Copy

P. FATHALIZADEH, M. DASHTIZAD, G. ASAADI TEHRANI, and M. DALIRI JOUPARI, “THE EFFECT OF VITRIFICATION ON PLURIPOTENCY SPECIFIC GENES, OCT4 AND NANOG IN MOUSE BLASTOCYSTS,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 28, no. 4, pp. 568–578, 2015, [Online]. Available: https://sid.ir/paper/248578/en

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