OPTIMIZATION OF CHIMERIC CHITINASE42 PROKARYOTIC EXPRESSION AND COMPARISON OF ITS CHITINASE ACTIVITY WITH CHIT42

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MATROUDI S.; ZAMANI M.R.; MOTALLEBI M.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Molecular and Cellular Researches Year:2015 | Volume:28 | Issue:2 Page(s): 279-289

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

837

Download:

Cites:

Information Journal Paper

Title

OPTIMIZATION OF CHIMERIC CHITINASE42 PROKARYOTIC EXPRESSION AND COMPARISON OF ITS CHITINASE ACTIVITY WITH CHIT42

Author(s)

MATROUDI S. | ZAMANI M.R. | MOTALLEBI M. | Issue Writer Certificate

Keywords

CHIMERIC CHITINASEQ2

PROKARYOTIC EXPRESSIONQ2

CHITIN BINDING DOMAINQ2

TAGUCHI METHODQ2

Abstract

Chitinases have the ability of chitin digestion that constitutes a main compound of the fungal cell wall, insect exoskeletons, and crustacean shells. Chitinase Chit42 from Trichoderma atroviride PTCC5220 is considered to play an important role in the biocontrol activity of this fungus against phytopathogenic fungi. The Chitin-Binding Domain (ChBD) of Serratia marcescens chitinase B was selected and fused to the fungal chitinase, T. atroviride Chit42 using SOEing PCR with overlapping primers. The chimeric fragment was cloned into PROKARYOTIC EXPRESSION vector (pET26b+) and transformed to E. coli BL21-DE3. Culture conditions for chimeric enzyme production by E. coli were optimized by Taguchi orthogonal array experimental design methodology. This approach facilitates the study of interaction of a large number of variables spanned by factors and their settings with a small number of experiments leading to considerable saving in time and cost for the process optimization. The objective of the current research was to determine the significant parameters on the production of CHIMERIC CHITINASE enzyme in the culture. The process variables were IPTG concentration, incubation time, and temperature. The total protein extraction of all experiments were carried out and analyzed by SDS-PAGE, Total lab and Qualitek-4 software. The optimal levels of the different factors for CHIMERIC CHITINASE production were 1mM IPTG, and 16 hours of incubation time at 28oC. IPTG concentration was the most important factor in the enzyme production.

Cites

No record.

References

No record.

Cite

APA: Copy

MATROUDI, S., ZAMANI, M.R., & MOTALLEBI, M.. (2015). OPTIMIZATION OF CHIMERIC CHITINASE42 PROKARYOTIC EXPRESSION AND COMPARISON OF ITS CHITINASE ACTIVITY WITH CHIT42. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 28(2), 279-289. SID. https://sid.ir/paper/248623/en

Vancouver: Copy

MATROUDI S., ZAMANI M.R., MOTALLEBI M.. OPTIMIZATION OF CHIMERIC CHITINASE42 PROKARYOTIC EXPRESSION AND COMPARISON OF ITS CHITINASE ACTIVITY WITH CHIT42. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2015;28(2):279-289. Available from: https://sid.ir/paper/248623/en

IEEE: Copy

S. MATROUDI, M.R. ZAMANI, and M. MOTALLEBI, “OPTIMIZATION OF CHIMERIC CHITINASE42 PROKARYOTIC EXPRESSION AND COMPARISON OF ITS CHITINASE ACTIVITY WITH CHIT42,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 28, no. 2, pp. 279–289, 2015, [Online]. Available: https://sid.ir/paper/248623/en

Related Journal Papers