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Information Journal Paper

Title

INDUCTION OF DIRECT SOMATIC EMBRYOGENESIS IN LEAF EXPLANTS OF ULMUS GLABRA

Pages

  885-894

Abstract

 Ulmus glabrais an economical important tree species found in forests. However it is endangered due to high susceptibility to Dutch Elm Disease. Therefore, an efficient micro propagation method is important for breeding and genetic improvement of elm. This is the first report on induction of direct SOMATIC EMBRYOGENESIS fromin vitro LEAF explants in elm. The first experiment conducted with MS medium supplemented with different concentrations of BA or Kin or TDZ under light condition. Second experiment, carried out with MS basal media supplemented with 200 mg l–1 casein hydro lysate and different levels of 2, 4-D and Kinetin in darkness. In third experiment 1.2 MS media supplemented with 200 mg l–1 casein hydro lysed and low levels of 2, 4-D in combination with Kin were used, and the effects of light were investigated. Globular somatic embryos were achieved directly just on small leaves of genotype 2 in experiment 3. Non-embryo genic calli were induced in other experiments. The frequency of embryogenesis in most of the treatments of experiment 3 was 100%. However, high number of embryos per explants was achieved in presence of 0.2 mgl-1 2, 4-D without Kin (60.48) and 0.4 mgl-1 2, 4-D combined with 0.2 mgl-1 Kin (47.76). Additionally, the percentage of embryogenesis and the average number of embryos per explants were higher in dark compared to the light condition.

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    APA: Copy

    MIRABBASI, S.M., HOSSEINPOUR, B., GHAEMMAGHAMI, S.A., & SANJABI, M.R.. (2015). INDUCTION OF DIRECT SOMATIC EMBRYOGENESIS IN LEAF EXPLANTS OF ULMUS GLABRA. JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 28(4), 885-894. SID. https://sid.ir/paper/248912/en

    Vancouver: Copy

    MIRABBASI S.M., HOSSEINPOUR B., GHAEMMAGHAMI S.A., SANJABI M.R.. INDUCTION OF DIRECT SOMATIC EMBRYOGENESIS IN LEAF EXPLANTS OF ULMUS GLABRA. JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2015;28(4):885-894. Available from: https://sid.ir/paper/248912/en

    IEEE: Copy

    S.M. MIRABBASI, B. HOSSEINPOUR, S.A. GHAEMMAGHAMI, and M.R. SANJABI, “INDUCTION OF DIRECT SOMATIC EMBRYOGENESIS IN LEAF EXPLANTS OF ULMUS GLABRA,” JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 28, no. 4, pp. 885–894, 2015, [Online]. Available: https://sid.ir/paper/248912/en

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