EFFECT OF CULTURE MEDIUMS AND LIGHT INTENSITY ON GROWTH AND CAROTENOIDES OF DUNALIELLA SALINA IN URMIA LAKE

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SALMANINEJAD M.

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Journal: Journal of Plant Research Year:2015 | Volume:28 | Issue:4 Page(s): 771-783

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Title

EFFECT OF CULTURE MEDIUMS AND LIGHT INTENSITY ON GROWTH AND CAROTENOIDES OF DUNALIELLA SALINA IN URMIA LAKE

Author(s)

SALMANINEJAD M. | Issue Writer Certificate

Keywords

CAROTENOIDEQ2

DUNALIELLA SALINAQ2

LIGHT INTENSITYQ2

SALTYQ2

URMIA LAKEQ1

Abstract

In the present research project effect of three culture medium and LIGHT INTENSITY on CAROTENOIDEs of algae, DUNALIELLA SALINA.In this study, Dunaliella species were collected from the lake and then was purified agar plate method. To study the effect of culture medium on the CAROTENOIDEs of this species six treatments in triplicateof each culture medium by salinity 1.03, 2.99, 4.96 M in LIGHT INTENSITY of 40, 120 and 200 micromol photon. m-2.s-1special culture module. Algae cells were counted regularly using Hemocitometry counting chamber in 3 replicates on daily basis. The growth curve was plotted out in Microsoft Excel software. The CAROTENOIDEs synthesized curve was plotted out in Microsoft Excel software and compared within treatments by means of two ways ANOVA Analysis. Specific growth rate showed significant differences in various treatments (P<0.05). The maximum growth rate (2.45 d⁻¹) was recorded in LIGHT INTENSITY of 40 μmol.photon.m⁻².s⁻¹and salinity 2.99 M and the minimum (0.94 d⁻¹) in 120 μmol.photon.m⁻².s⁻¹ at salinity of 1.03 M. The obtained results showed that increase light in culture medium 1.03 M from 40 to 200 mmol photon m⁻².s⁻¹ correlates with decrease in cell concentration and growth rate in D. salina. While, increase in light intensities in culture medium 2.99 M from 40 to 200 μmolphoton.m⁻².s⁻¹ resulted in reduction of cell concentration and growth rate in this algae. Analysis of Variance results obtained showed a significant variation between different treatments of salinity and light on scale CAROTENOIDEs has a direct relation with salinity. and LIGHT INTENSITY and Interaction (P<0.05). Significant interaction between the different levels of light and salinity showed that the greatest scale of CAROTENOIDEs in the culture medium is in LIGHT INTENSITY 200 mm and salt concentration 2.99 M The amount 5.07 mg.ml-1 and least scale of CAROTENOIDEs in LIGHT INTENSITY 120 mm and salt concentration 1.03 M The amount 1.02 mg.ml-1. The obtained results showed that increase in LIGHT INTENSITY from 40 to 200 mm resulted in increase Synthesis of CAROTENOIDEs in D. salina. and The influence best of salt concentration pro production of CAROTENOIDEs is salinity of 2.99 M.

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APA: Copy

SALMANINEJAD, M.. (2015). EFFECT OF CULTURE MEDIUMS AND LIGHT INTENSITY ON GROWTH AND CAROTENOIDES OF DUNALIELLA SALINA IN URMIA LAKE. JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 28(4), 771-783. SID. https://sid.ir/paper/248920/en

Vancouver: Copy

SALMANINEJAD M.. EFFECT OF CULTURE MEDIUMS AND LIGHT INTENSITY ON GROWTH AND CAROTENOIDES OF DUNALIELLA SALINA IN URMIA LAKE. JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2015;28(4):771-783. Available from: https://sid.ir/paper/248920/en

IEEE: Copy

M. SALMANINEJAD, “EFFECT OF CULTURE MEDIUMS AND LIGHT INTENSITY ON GROWTH AND CAROTENOIDES OF DUNALIELLA SALINA IN URMIA LAKE,” JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 28, no. 4, pp. 771–783, 2015, [Online]. Available: https://sid.ir/paper/248920/en

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