The optimization of gene transfer to tomato and the study of expression possibility of salt-tolerance gene (SOS3)

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Mohajel Kazemi Elham; Pazhouhandeh Maghsoud; JONOUBI PARISA; KAZEMIAN MINA

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Journal: NOVA BIOLOGICA REPERTA Year:2020 | Volume:7 | Issue:1 Page(s): 76-84

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Title

The optimization of gene transfer to tomato and the study of expression possibility of salt-tolerance gene (SOS3)

Author(s)

Mohajel Kazemi Elham | Pazhouhandeh Maghsoud | JONOUBI PARISA | KAZEMIAN MINA | Issue Writer Certificate

Keywords

agrobacteriumQ2

cotyledonQ2

hypocotylQ1

regenerationQ2

transgeneQ2

Abstract

One of the main strategies to improve plant tolerance is the expression of stress-induced genes, which play a significant role in the ionic balance of plants. SOS3 is one of the important components of SOS-regulated ionic homeostasis pathway. Therefore, the expression of this gene could be an important step towards producing salt-resistant plants. In this work, we have transformed tomato (Solanum lycopersicum) by agrobacterium (GV3101 and LBA4404) containing plasmids with SOS3 genes. The maximum regeneration rate was determined in cotyledons of CH genotype. The simultaneous use of cotyledons and hypocotyls in the culture medium had the best outcome. In addition, the best time was found to be one day after inoculation. Also, the best transgenic variety was detected for agrobacterium GV3101, which can be attributed to the interaction between the genus agrobacterium and the tomato variety. Transgenic plants were transferred to a culture medium containing sequestrene, which caused the acceleration of the seedling growth in particular. The presence of the SOS3 in the transgenic plants was verified by PCR and RT-PCR methods.

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APA: Copy

Mohajel Kazemi, Elham, Pazhouhandeh, Maghsoud, JONOUBI, PARISA, & KAZEMIAN, MINA. (2020). The optimization of gene transfer to tomato and the study of expression possibility of salt-tolerance gene (SOS3). NOVA BIOLOGICA REPERTA, 7(1 ), 76-84. SID. https://sid.ir/paper/250464/en

Vancouver: Copy

Mohajel Kazemi Elham, Pazhouhandeh Maghsoud, JONOUBI PARISA, KAZEMIAN MINA. The optimization of gene transfer to tomato and the study of expression possibility of salt-tolerance gene (SOS3). NOVA BIOLOGICA REPERTA[Internet]. 2020;7(1 ):76-84. Available from: https://sid.ir/paper/250464/en

IEEE: Copy

Elham Mohajel Kazemi, Maghsoud Pazhouhandeh, PARISA JONOUBI, and MINA KAZEMIAN, “The optimization of gene transfer to tomato and the study of expression possibility of salt-tolerance gene (SOS3),” NOVA BIOLOGICA REPERTA, vol. 7, no. 1 , pp. 76–84, 2020, [Online]. Available: https://sid.ir/paper/250464/en

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