Sequencing of the rus gene before and after the mutation with DES in the bacterial Acidithiobacillus sp. FJ2

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Farahmand Somayeh; FATEMI FAEZEH; HAJIHOSSEINI REZA

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Journal: NOVA BIOLOGICA REPERTA Year:2019 | Volume:6 | Issue:1 Page(s): 50-60

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Title

Sequencing of the rus gene before and after the mutation with DES in the bacterial Acidithiobacillus sp. FJ2

Author(s)

Farahmand Somayeh | FATEMI FAEZEH | HAJIHOSSEINI REZA | Issue Writer Certificate

Keywords

bioleachingQ1

electron transportQ2

microorganismQ2

proteinQ1

random mutationQ1

Abstract

In Acidithiobacillus ferrooxidans, the proteins present in the electron transfer pathway cause ferrous iron oxidation which leads to uranium extraction. The relationship between gene sequence and uranium extraction has not been investigated yet. Based on the changes in uranium extraction, the changes of rus gene sequence can reveal the direct and accurate role of this protein. For this purpose, a random mutation was induced in native Acidithiobacillus sp. FJ2 by two doses of 0. 8% and 1% of DES. Then, the bacteria was transferred into a medium which contained 50% uranium ore to carry out the bioleaching process. After measuring the amount of the extracted uranium, iron, Eh and pH, genomic DNA was extracted to investigate the rusticyanin gene (rus) sequence sent for sequencing after performing PCR. Then, the wild-type gene sequence was compared with the mutant by Bioedit v7. 2. 5 software. The results showed that uranium extraction increased by mutant bacteria with DES 1% between 7-11 days in comparison with wild bacteria. However, there has been no change in the functional areas of the rusticyanin gene. It seems that DES affected other effective genes in the electron transport chain or regulatory areas, which required further studies.

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APA: Copy

Farahmand, Somayeh, FATEMI, FAEZEH, & HAJIHOSSEINI, REZA. (2019). Sequencing of the rus gene before and after the mutation with DES in the bacterial Acidithiobacillus sp. FJ2. NOVA BIOLOGICA REPERTA, 6(1 ), 50-60. SID. https://sid.ir/paper/250515/en

Vancouver: Copy

Farahmand Somayeh, FATEMI FAEZEH, HAJIHOSSEINI REZA. Sequencing of the rus gene before and after the mutation with DES in the bacterial Acidithiobacillus sp. FJ2. NOVA BIOLOGICA REPERTA[Internet]. 2019;6(1 ):50-60. Available from: https://sid.ir/paper/250515/en

IEEE: Copy

Somayeh Farahmand, FAEZEH FATEMI, and REZA HAJIHOSSEINI, “Sequencing of the rus gene before and after the mutation with DES in the bacterial Acidithiobacillus sp. FJ2,” NOVA BIOLOGICA REPERTA, vol. 6, no. 1 , pp. 50–60, 2019, [Online]. Available: https://sid.ir/paper/250515/en

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