USING EFFECTIVE COMPOUNDS ON SPERM CHROMATIN STRUCTURE IN INTRACYTOPLASMIC SPERM INJECTION (ICSI) PROCEDURE

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GOLESTANFAR A.; SHIRAZI A.; SHAMS ESFANDABADI N.; AHMADI E.

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Journal: IRANIAN JOURNAL OF VETERINARY CLINICAL SCIENCES Year:2017 | Volume:11 | Issue:1 Page(s): 99-107

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Information Journal Paper

Title

USING EFFECTIVE COMPOUNDS ON SPERM CHROMATIN STRUCTURE IN INTRACYTOPLASMIC SPERM INJECTION (ICSI) PROCEDURE

Author(s)

GOLESTANFAR A. | SHIRAZI A. | SHAMS ESFANDABADI N. | AHMADI E. | Issue Writer Certificate

Keywords

SPERM DECONDENSATIONQ1

OOCYTE ACTIVATIONQ1

ICSIQ1

GLUTATHIONEQ1

SPERM EXTRACTQ1

Abstract

Considering the low percentages of sperm chromatin decondensation and male pronuclear formation following intracytoplasmic sperm injection (ICSI) in ovine species which decrease the chance of normal fertilization, the current study was designed to assess the effects of some methods of sperm pretreatment on male pronuclear (MPN) formation and OOCYTE ACTIVATION in ovine ICSI procedure. The experimental groups of this study were comprised of: I) ICSI of untreated sperm (control), II) ICSI of pretreated sperm with GSH+Hep (GSH+Hep), III) ICSI of pretreated sperm with GSH+Hep coinjected with GSH (GSH+Hep+inj.GSH), and IV) ICSI of pretreated sperm with GSH+Hep coinjected with SPERM EXTRACT (GSH+Hep+inj.SE). The injected oocytes were activated with Io+6-DAMP and after 16h, they were examined for prenuclear formation. The MPN formation in GSH+Hep+inj.GSH was higher (P<0.001) than other groups. The rate of activated ICSI oocytes in GSH+Hep group was higher (P<0.05) than other groups. In conclusion, ICSI of pretreated sperm with GSH+Hep accompanied with GSH injection has dramatic effect on MPN formation in ovine species.

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APA: Copy

GOLESTANFAR, A., SHIRAZI, A., SHAMS ESFANDABADI, N., & AHMADI, E.. (2017). USING EFFECTIVE COMPOUNDS ON SPERM CHROMATIN STRUCTURE IN INTRACYTOPLASMIC SPERM INJECTION (ICSI) PROCEDURE. IRANIAN JOURNAL OF VETERINARY CLINICAL SCIENCES, 11(1), 99-107. SID. https://sid.ir/paper/260129/en

Vancouver: Copy

GOLESTANFAR A., SHIRAZI A., SHAMS ESFANDABADI N., AHMADI E.. USING EFFECTIVE COMPOUNDS ON SPERM CHROMATIN STRUCTURE IN INTRACYTOPLASMIC SPERM INJECTION (ICSI) PROCEDURE. IRANIAN JOURNAL OF VETERINARY CLINICAL SCIENCES[Internet]. 2017;11(1):99-107. Available from: https://sid.ir/paper/260129/en

IEEE: Copy

A. GOLESTANFAR, A. SHIRAZI, N. SHAMS ESFANDABADI, and E. AHMADI, “USING EFFECTIVE COMPOUNDS ON SPERM CHROMATIN STRUCTURE IN INTRACYTOPLASMIC SPERM INJECTION (ICSI) PROCEDURE,” IRANIAN JOURNAL OF VETERINARY CLINICAL SCIENCES, vol. 11, no. 1, pp. 99–107, 2017, [Online]. Available: https://sid.ir/paper/260129/en

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