EFFECT OF HEAT STRESS ON NITRIC OXIDE PRODUCTION AND GENE EXPRESSION OF TWO NITRIC OXIDE SYNTHASE ENZYMES IN THE OVINE SERTOLI CELLS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

KADIVAR A.; HASSANPOUR H.; MIRSHOKRAEI P.; SALEHINEJAD F.; AFZALI A.; NAZARI H.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF VETERINARY CLINICAL SCIENCES Year:2016 | Volume:10 | Issue:1 Page(s): 47-54

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,062

Download:

Cites:

Information Journal Paper

Title

EFFECT OF HEAT STRESS ON NITRIC OXIDE PRODUCTION AND GENE EXPRESSION OF TWO NITRIC OXIDE SYNTHASE ENZYMES IN THE OVINE SERTOLI CELLS

Author(s)

Keywords

HEAT STRESSQ1

NITRIC OXIDEQ2

NITRIC OXIDE SYNTHASEQ1

SERTOLI CELLQ2

Abstract

The aim of the present study was to evaluate gene expression of constitutive and inductive NITRIC OXIDE synthase enzymes (cNOS, iNOS) and measurement of NITRIC OXIDE metabolite in ovine SERTOLI CELLs under HEAT STRESS. Testes of ten male lambs were provided from abattoir and then transported to the lab for separating of SERTOLI CELLs. HEAT STRESS was challenged in three groups which consist of control (temperature of 32oC), mild HEAT STRESS (temperature of 39˚C) and severe HEAT STRESS (temperature of 42oC). The amount of nitrite (nitric oxide metabolite) was significantly higher in 42 oC-group than control (p<0.05). The mRNAs of constitutive NITRIC OXIDE synthase gene in 39˚C-group and inductive NITRIC OXIDE synthase gene in 42 oC-group were significantly tha that of control (p<0.05). HEAT STRESS is a destructive agent in the viability and function of SERTOLI CELLs. Mechanism of this effect is via high production of free radicals such as NITRIC OXIDE, which could cause serious damages and make apoptosis. In this research, SERTOLI CELLs of sheep were introduced as suitable model for evaluation of NITRIC OXIDE production and gene expression of NITRIC OXIDE synthase enzymes.

Cites

No record.

References

No record.

Cite

APA: Copy

KADIVAR, A., HASSANPOUR, H., MIRSHOKRAEI, P., SALEHINEJAD, F., AFZALI, A., & NAZARI, H.. (2016). EFFECT OF HEAT STRESS ON NITRIC OXIDE PRODUCTION AND GENE EXPRESSION OF TWO NITRIC OXIDE SYNTHASE ENZYMES IN THE OVINE SERTOLI CELLS. IRANIAN JOURNAL OF VETERINARY CLINICAL SCIENCES, 10(1), 47-54. SID. https://sid.ir/paper/260142/en

Vancouver: Copy

KADIVAR A., HASSANPOUR H., MIRSHOKRAEI P., SALEHINEJAD F., AFZALI A., NAZARI H.. EFFECT OF HEAT STRESS ON NITRIC OXIDE PRODUCTION AND GENE EXPRESSION OF TWO NITRIC OXIDE SYNTHASE ENZYMES IN THE OVINE SERTOLI CELLS. IRANIAN JOURNAL OF VETERINARY CLINICAL SCIENCES[Internet]. 2016;10(1):47-54. Available from: https://sid.ir/paper/260142/en

IEEE: Copy

A. KADIVAR, H. HASSANPOUR, P. MIRSHOKRAEI, F. SALEHINEJAD, A. AFZALI, and H. NAZARI, “EFFECT OF HEAT STRESS ON NITRIC OXIDE PRODUCTION AND GENE EXPRESSION OF TWO NITRIC OXIDE SYNTHASE ENZYMES IN THE OVINE SERTOLI CELLS,” IRANIAN JOURNAL OF VETERINARY CLINICAL SCIENCES, vol. 10, no. 1, pp. 47–54, 2016, [Online]. Available: https://sid.ir/paper/260142/en

Related Journal Papers