EFFECT OF VITAMIN E ON CELL VIABILITY AND NITRIC OXIDE RELEASE

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AHMADI KAZEM; ARAB SALMANI F.

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Journal: KOWSAR MEDICAL JOURNAL Year:2006 | Volume:11 | Issue:2 Page(s): 155-161

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Title

EFFECT OF VITAMIN E ON CELL VIABILITY AND NITRIC OXIDE RELEASE

Author(s)

AHMADI KAZEM | ARAB SALMANI F. | Issue Writer Certificate

Keywords

MACROPHAGEQ3

CELL VIABILITYQ3

CELL DEATHQ3

VITAMIN E AND NITRIC OXIDEQ3

Abstract

Objective: Evaluation of the effect of Vitamin E as an antioxidant on CELL VIABILITY and NO release by peritoneal MACROPHAGEs.Material and Method: The peritoneal MACROPHAGEs were extracted by washing the mice peritoneal with 5 ml ice-cold RPMI medium. Cells were washed, counted and plated out at 1x105 cells in 96 well plates. Plates were incubated at 37°C, 5% CO2 in air for 2 hours. The nonadherent cells were removed by washing with warm PBS. The adherent cells were then cultured at above condition in phenol red free medium in the presence or absence of Vitamin E/LPS up to 6 day. The culture medium was replaced in different days. CELL VIABILITY was checked with trypan blue, nitrite was measured as an indicator of NO by Griess method.Results: The results showed that CELL DEATH is higher in normal condition with days of incubation comparing with cells receiving vitamin E (p<0.02). In the absence of vitamin E maximum NO was produced after 48 hours. Medium replacement has enhanced NO release and medium replacement in early days had the best effect. Vitamin E enhanced CELL VIABILITY and reduced No release. Conclusions: Reduction of NO production by times of incubation could be as a result of CELL DEATH, finishing medium or the toxicity of metabolite. Enhancement of CELL VIABILITY, reduction of NO release in the presence of Vitamin E shows that Vitamin E affect CELL DEATH in tissue culture through No release.

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APA: Copy

AHMADI, KAZEM, & ARAB SALMANI, F.. (2006). EFFECT OF VITAMIN E ON CELL VIABILITY AND NITRIC OXIDE RELEASE. KOWSAR MEDICAL JOURNAL, 11(2), 155-161. SID. https://sid.ir/paper/33031/en

Vancouver: Copy

AHMADI KAZEM, ARAB SALMANI F.. EFFECT OF VITAMIN E ON CELL VIABILITY AND NITRIC OXIDE RELEASE. KOWSAR MEDICAL JOURNAL[Internet]. 2006;11(2):155-161. Available from: https://sid.ir/paper/33031/en

IEEE: Copy

KAZEM AHMADI, and F. ARAB SALMANI, “EFFECT OF VITAMIN E ON CELL VIABILITY AND NITRIC OXIDE RELEASE,” KOWSAR MEDICAL JOURNAL, vol. 11, no. 2, pp. 155–161, 2006, [Online]. Available: https://sid.ir/paper/33031/en

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