CHEMOMETRIC APPROACHES TO THE OPTIMIZATION OF SIMULANEOUS SEPARATION OF SIX ANTIEPILEPTIC DRUGS: INVESTIGATION OF PHYSICAL AND CHEMICAL INTERFERENCE IN SERUM DURING MEASUREMENT

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EBRAHIMI POUNEH; PORMORAD F.; HONARY S.

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Journal: Nashrieh Shimi va Mohandesi Shimi Iran Year:2010 | Volume:29 | Issue:3 Page(s): 111-120

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Title

CHEMOMETRIC APPROACHES TO THE OPTIMIZATION OF SIMULANEOUS SEPARATION OF SIX ANTIEPILEPTIC DRUGS: INVESTIGATION OF PHYSICAL AND CHEMICAL INTERFERENCE IN SERUM DURING MEASUREMENT

Author(s)

EBRAHIMI POUNEH | PORMORAD F. | HONARY S. | Issue Writer Certificate

Keywords

ANTICONVULSANT DRUGSQ3

EXPERIMENTAL DESIGNQ1

MULTIPLE LINEAR REGRESSIONQ3

CHEMOMETRICS APPROACHESQ3

OPTIMIZATIONQ2

Abstract

Determination of the drug’s serum concentration has an important role in pharmacokinetic and toxicity studies. There is a significant relation between serum level and drug effects which can helps to find a therapeutic protocol. The objective of this study is to investigate optimum condition to separate 6 anticonvulsant agents (primidone, phenobarbital, phenytoin, carbamazepine, ethosuximide and diazepam) by chemometrix method in patient’s serum. Also, the impact of physical and chemical disturbances due to serum environment on the calibration curve in simultaneous measurement of six compounds has been evaluated. Chemometrix methods such as EXPERIMENTAL DESIGN, multivariable analysis and Parto multifactorial decision was used to achieve an optimum analysis time and suitable resolution of neighborhood peaks. pH and organic solvent were selected as the variables. Serum drug’s amount was determined via calibration graph. The analysis method showed the most important factors affecting on retention time of the drugs was methanol percentage in the mobile phase. The involvement of other variables was not significant. The interaction of two factors in retention model exhibited the necessity of carrying out a multifactorial analysis method to separate anticonvusant drugs in RP-HPLC. Our study showed the optimum condition in pH = 26 and V= 60%. There was no effect from serum physical and chemical factors on the determination of drugs in this study in regard to phenobarbital, phenytoin and carbamazepine. Therefore this model can be used for separation of these drugs in mixture but this method should be changed to standard addition in order to measure ethosuximide and diazepam.

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APA: Copy

EBRAHIMI, POUNEH, PORMORAD, F., & HONARY, S.. (2010). CHEMOMETRIC APPROACHES TO THE OPTIMIZATION OF SIMULANEOUS SEPARATION OF SIX ANTIEPILEPTIC DRUGS: INVESTIGATION OF PHYSICAL AND CHEMICAL INTERFERENCE IN SERUM DURING MEASUREMENT. NASHRIEH SHIMI VA MOHANDESI SHIMI IRAN (PERSIAN), 29(3), 111-120. SID. https://sid.ir/paper/26161/en

Vancouver: Copy

EBRAHIMI POUNEH, PORMORAD F., HONARY S.. CHEMOMETRIC APPROACHES TO THE OPTIMIZATION OF SIMULANEOUS SEPARATION OF SIX ANTIEPILEPTIC DRUGS: INVESTIGATION OF PHYSICAL AND CHEMICAL INTERFERENCE IN SERUM DURING MEASUREMENT. NASHRIEH SHIMI VA MOHANDESI SHIMI IRAN (PERSIAN)[Internet]. 2010;29(3):111-120. Available from: https://sid.ir/paper/26161/en

IEEE: Copy

POUNEH EBRAHIMI, F. PORMORAD, and S. HONARY, “CHEMOMETRIC APPROACHES TO THE OPTIMIZATION OF SIMULANEOUS SEPARATION OF SIX ANTIEPILEPTIC DRUGS: INVESTIGATION OF PHYSICAL AND CHEMICAL INTERFERENCE IN SERUM DURING MEASUREMENT,” NASHRIEH SHIMI VA MOHANDESI SHIMI IRAN (PERSIAN), vol. 29, no. 3, pp. 111–120, 2010, [Online]. Available: https://sid.ir/paper/26161/en

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