OPTIMIZATOIN OF PRODUCTION OF 6-APA FROM E.COLI ATCC11105 IMMOBILIZED IN CALCIUM ALGINATE

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VAZIRI YAZDI A.; OTADY M.; SOLTANI F.; SEYF KORDI A.A.; KHEYR ALOMOUM A.

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Journal: Nashrieh Shimi va Mohandesi Shimi Iran Year:2006 | Volume:25 | Issue:2 Page(s): 97-104

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Information Journal Paper

Title

OPTIMIZATOIN OF PRODUCTION OF 6-APA FROM E.COLI ATCC11105 IMMOBILIZED IN CALCIUM ALGINATE

Author(s)

Keywords

CELL IMMOBILIZATIONQ4

PGAQ4

ALGINATEQ2

6-APAQ4

Abstract

Escherichia coli ATCC11105 was immobilized in calcium ALGINATE beads to catalyze production of 6-aminopenicillanic acid (6-APA) from penicillin G (potassium salt). Cell concentration in beads was found to be an important factor influencing the maximum velocity and specific activity of beads, and the optimum value of ALGINATE to cell suspension ratio was 1.33. The effect of other important factors such as substrate penicillin G concentration, buffer pH, and the temperature on immobilized cell performance were also studied, and the optimal conditions were found to be 2% substrate, pH 7.5, and 45 °C, respectively. Under the optimal reaction conditions, the immobilized cells retained 90% of the initial activity after 25 repeated batches of 6 hours each.

Cites

بهینه سازی تهیه فراصوتی و بررسی تورم ژل ابرجاذب با شبکه ساز متیلن بیس آکریل آمید

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APA: Copy

VAZIRI YAZDI, A., OTADY, M., SOLTANI, F., SEYF KORDI, A.A., & KHEYR ALOMOUM, A.. (2006). OPTIMIZATOIN OF PRODUCTION OF 6-APA FROM E.COLI ATCC11105 IMMOBILIZED IN CALCIUM ALGINATE. NASHRIEH SHIMI VA MOHANDESI SHIMI IRAN (PERSIAN), 25(2), 97-104. SID. https://sid.ir/paper/26446/en

Vancouver: Copy

VAZIRI YAZDI A., OTADY M., SOLTANI F., SEYF KORDI A.A., KHEYR ALOMOUM A.. OPTIMIZATOIN OF PRODUCTION OF 6-APA FROM E.COLI ATCC11105 IMMOBILIZED IN CALCIUM ALGINATE. NASHRIEH SHIMI VA MOHANDESI SHIMI IRAN (PERSIAN)[Internet]. 2006;25(2):97-104. Available from: https://sid.ir/paper/26446/en

IEEE: Copy

A. VAZIRI YAZDI, M. OTADY, F. SOLTANI, A.A. SEYF KORDI, and A. KHEYR ALOMOUM, “OPTIMIZATOIN OF PRODUCTION OF 6-APA FROM E.COLI ATCC11105 IMMOBILIZED IN CALCIUM ALGINATE,” NASHRIEH SHIMI VA MOHANDESI SHIMI IRAN (PERSIAN), vol. 25, no. 2, pp. 97–104, 2006, [Online]. Available: https://sid.ir/paper/26446/en

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