Applying a system-oriented method for culture medium design to improve bioethanol production by Synechocystis

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Babazadegan Mohammad Ali; MOTAMEDIAN EHSAN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: APPLIED RESEARCH IN CHEMICAL POLYMER ENGINEERING Year:2019 | Volume:3 | Issue:2 Page(s): 55-67

Download Full-Text

Persian Verion

View:

491

Download:

Cites:

Information Journal Paper

Title

Applying a system-oriented method for culture medium design to improve bioethanol production by Synechocystis

Author(s)

Babazadegan Mohammad Ali | MOTAMEDIAN EHSAN | Issue Writer Certificate

Keywords

Regulatory culture medium bio-fuel

activator and inhibitor Brenda database

design of experiment

genom-scale metabolic

model Synechocystis

Abstract

Research subject: The use of genetic engineering tools to produce industrial strains, especially from non-model microorganisms such as cyanobacteria, is always subject to limitations. Research approach: In this research, a system-oriented method was used to design a culture medium instead of strain designing and its ability to increase ethanol production by Synechocystis sp. PCC 6803 was experimentally evaluated. In this method, compounds are added to the medium to regulate the activity of target enzymes not for the purpose of being consumed by the cells, and thus, the designed culture medium eliminates the intracellular constraints on the production. A metabolic model was used to determine the minimum level of ethanol production and to identify genes that increase or decrease of their expression increase this minimum level. Then, regulators of the enzyme expressed by the target genes were extracted from the Brenda database and their effect on the production was evaluated experimentally and design of experiment was performed to optimize the concentration of the selected compounds. Main results: Among the compounds identified, two inhibitors (salicylic acid and mercuric chloride) and one activator (pyruvate) were selected to be added to the medium and their concentration was optimized using the central composite design method. The proposed regulatory medium increased the production of ethanol from 352 to 1116 mg/l, indicating the effectiveness of the added regulatory compounds on the cyanobacteria metabolism. The proposed systemoriented method can be used to design medium culture for other important bio-products such as recombinant proteins.

Cites

No record.

References

No record.

Cite

APA: Copy

Babazadegan, Mohammad Ali, & MOTAMEDIAN, EHSAN. (2019). Applying a system-oriented method for culture medium design to improve bioethanol production by Synechocystis. APPLIED RESEARCH IN CHEMICAL POLYMER ENGINEERING, 3(2), 55-67. SID. https://sid.ir/paper/267788/en

Vancouver: Copy

Babazadegan Mohammad Ali, MOTAMEDIAN EHSAN. Applying a system-oriented method for culture medium design to improve bioethanol production by Synechocystis. APPLIED RESEARCH IN CHEMICAL POLYMER ENGINEERING[Internet]. 2019;3(2):55-67. Available from: https://sid.ir/paper/267788/en

IEEE: Copy

Mohammad Ali Babazadegan, and EHSAN MOTAMEDIAN, “Applying a system-oriented method for culture medium design to improve bioethanol production by Synechocystis,” APPLIED RESEARCH IN CHEMICAL POLYMER ENGINEERING, vol. 3, no. 2, pp. 55–67, 2019, [Online]. Available: https://sid.ir/paper/267788/en

Related Journal Papers