STABILIZING AND SUICIDE-PEROXIDE PROTECTING EFFECT OF NI2+ ON HORSERADISH PEROXIDASE

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NAZARI K.; MAHMOUDI A.; MOHEBI A.; MOUSAVI MOVAHEDI ALI AKBAR; KHODAFARIN R.

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Paper Information

Journal: JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS) Year:2005 | Volume:2 | Issue:3 Page(s): 232-237

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Information Journal Paper

Title

STABILIZING AND SUICIDE-PEROXIDE PROTECTING EFFECT OF NI2+ ON HORSERADISH PEROXIDASE

Author(s)

Keywords

HORSERADISH PEROXIDASE

SUICIDE INACTIVATION

ENZYME PROTECTION

NI2++

Abstract

The present research discusses the structure stabilizing and protecting effects of NI2++ against suicide-peroxide inactivation of HORSERADISH PEROXIDASE (HRP). SUICIDE INACTIVATION of HRP by hydrogen peroxide (3 mM) was monitored by measuring change in the absorbance of the colored product (tetraguaiacol) of the catalytic reaction cycle at 470 nm. Progress curves of the catalytic reaction cycle were obtained at 27 oC, phosphate buffer (5 mM), pH 7.0. The corresponding kinetic parameters (e.g., initial enzyme activity (a o) and the apparent rate constant (ki) of SUICIDE INACTIVATION of HRP by peroxide) were evaluated using a kinetic equation derived in this study. Comparative activatory and inhibitory effects of NI2++ on the kinetics of suicide-peroxide inactivation of HRP are discussed.

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APA: Copy

NAZARI, K., MAHMOUDI, A., MOHEBI, A., MOUSAVI MOVAHEDI, ALI AKBAR, & KHODAFARIN, R.. (2005). STABILIZING AND SUICIDE-PEROXIDE PROTECTING EFFECT OF NI2+ ON HORSERADISH PEROXIDASE . JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS), 2(3), 232-237. SID. https://sid.ir/paper/282051/en

Vancouver: Copy

NAZARI K., MAHMOUDI A., MOHEBI A., MOUSAVI MOVAHEDI ALI AKBAR, KHODAFARIN R.. STABILIZING AND SUICIDE-PEROXIDE PROTECTING EFFECT OF NI2+ ON HORSERADISH PEROXIDASE . JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS)[Internet]. 2005;2(3):232-237. Available from: https://sid.ir/paper/282051/en

IEEE: Copy

K. NAZARI, A. MAHMOUDI, A. MOHEBI, ALI AKBAR MOUSAVI MOVAHEDI, and R. KHODAFARIN, “STABILIZING AND SUICIDE-PEROXIDE PROTECTING EFFECT OF NI2+ ON HORSERADISH PEROXIDASE ,” JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS), vol. 2, no. 3, pp. 232–237, 2005, [Online]. Available: https://sid.ir/paper/282051/en

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