IMMOBILIZATION OF LACTATE AS AN ELECTROACTIVE INDICATOR ON PENCIL GRAPHITE ELECTRODE FOR THE DEVELOPMENT OF A NEW ELECTROCHEMICAL BIOSENSOR FOR THE DETECTION OF LACTATE DEHYDROGENASE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MAJIDI M.R.; GHOLIZADEH S.; HEJAZI M.S.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS) Year:2011 | Volume:8 | Issue:1 Page(s): 59-67

Download Full-Text

View:

354

Download:

128

Cites:

Information Journal Paper

Title

IMMOBILIZATION OF LACTATE AS AN ELECTROACTIVE INDICATOR ON PENCIL GRAPHITE ELECTRODE FOR THE DEVELOPMENT OF A NEW ELECTROCHEMICAL BIOSENSOR FOR THE DETECTION OF LACTATE DEHYDROGENASE

Author(s)

MAJIDI M.R. | GHOLIZADEH S. | HEJAZI M.S. | Issue Writer Certificate

Keywords

LACTATE DEHYDROGENASE

LACTATE

SQUARE WAVE VOLTAMMETRY

PENCIL GRAPHITE ELECTRODE

NAD+

Abstract

A biosensor was developed for the detection of LACTATE DEHYDROGENASE (LDH) enzyme using a LACTATE modified PENCIL GRAPHITE ELECTRODE (PGE). The sensor relies on the immobilization of the LACTATE on PGE, and LDH detection is based on the decrease of LACTATE peak current following oxidation to pyruvate in the presence of LDH. Square wave voltammetric technique was used for the assay of signals in the range of -0.6 to 0.8 V and a frequency of 25 Hz for the determination of LDH. The dependence of the response was investigated in terms of reaction time, washing time and LDH and NAD+amounts. Also, the electrochemical behavior of LDH treatment on the LACTATE modified PGE was studied. The electrode showed good selectivity, repeatability and an operational stability of about 90% of its original response for two weeks. Moreover, the sensor displayed a linear response range from 0.36- 2.13 U ml-1 for LDH with a detection limit of 0.16 U ml-1. The response time of the LDH-treated LACTATE modified PGE was found to be 2s. The relative standard deviation (RSD) obtained was 3.5% (for LDH 0.71 U ml-1 and n=3).

Cites

No record.

References

No record.

Cite

APA: Copy

MAJIDI, M.R., GHOLIZADEH, S., & HEJAZI, M.S.. (2011). IMMOBILIZATION OF LACTATE AS AN ELECTROACTIVE INDICATOR ON PENCIL GRAPHITE ELECTRODE FOR THE DEVELOPMENT OF A NEW ELECTROCHEMICAL BIOSENSOR FOR THE DETECTION OF LACTATE DEHYDROGENASE. JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS), 8(1), 59-67. SID. https://sid.ir/paper/282515/en

Vancouver: Copy

MAJIDI M.R., GHOLIZADEH S., HEJAZI M.S.. IMMOBILIZATION OF LACTATE AS AN ELECTROACTIVE INDICATOR ON PENCIL GRAPHITE ELECTRODE FOR THE DEVELOPMENT OF A NEW ELECTROCHEMICAL BIOSENSOR FOR THE DETECTION OF LACTATE DEHYDROGENASE. JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS)[Internet]. 2011;8(1):59-67. Available from: https://sid.ir/paper/282515/en

IEEE: Copy

M.R. MAJIDI, S. GHOLIZADEH, and M.S. HEJAZI, “IMMOBILIZATION OF LACTATE AS AN ELECTROACTIVE INDICATOR ON PENCIL GRAPHITE ELECTRODE FOR THE DEVELOPMENT OF A NEW ELECTROCHEMICAL BIOSENSOR FOR THE DETECTION OF LACTATE DEHYDROGENASE,” JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS), vol. 8, no. 1, pp. 59–67, 2011, [Online]. Available: https://sid.ir/paper/282515/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops