SOLID-PHASE EXTRACTION OF L-TRYPTOPHAN FROM FOOD SAMPLES UTILIZING A LAYERED DOUBLE HYDROXIDE NANO-SORBENT PRIOR TO ITS DETERMINATION BY SPECTROFLUOROMETRY

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ABDOLMOHAMMAD ZADEH HOSSEIN; HAMMAMI OSKOOYI SEYED MORTEZA

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Journal Paper

Paper Information

Journal: JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS) Year:2015 | Volume:12 | Issue:6 Page(s): 1115-1122

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Title

SOLID-PHASE EXTRACTION OF L-TRYPTOPHAN FROM FOOD SAMPLES UTILIZING A LAYERED DOUBLE HYDROXIDE NANO-SORBENT PRIOR TO ITS DETERMINATION BY SPECTROFLUOROMETRY

Author(s)

ABDOLMOHAMMAD ZADEH HOSSEIN | HAMMAMI OSKOOYI SEYED MORTEZA

Keywords

L-TRYPTOPHAN

SOLID-PHASE EXTRACTION

NANO-SORBENT

LAYERED DOUBLE HYDROXIDE

SPECTROFLUOROMETRY

FOOD SAMPLES

Abstract

In this research, a simple method was developed for the separation and determination of L-TRYPTOPHAN in aqueous solutions. The method consists of a SOLID-PHASE EXTRACTION (SPE) cleanup system utilizing a column packed with nickel–aluminum LAYERED DOUBLE HYDROXIDE as a NANO-SORBENT, followed by a spectrofluorometric detection step. Some factors influencing the SPE efficiency, including pH, the sample flow rate, the elution conditions and the amount of NANO-SORBENT were evaluated and optimized. The selectivity of the presented SPE method with regard to L-TRYPTOPHAN was studied in the presence of other amino acids and potentially interfering ions. Under optimum conditions, the calibration graph was linear in the range of 0.1-120.0 mg L-1 with a correlation coefficient of 0.9975. The limit of detection and relative standard deviation for L-TRYPTOPHAN were 0.01 mg L-1 and 0.62 %, respectively. The maximum sorption capacity of the sorbent for L-TRYPTOPHAN was 37.6 mg g-1. The method was successfully applied to determine L-TRYPTOPHAN in several FOOD SAMPLES with recoveries in the range of 96.2-104.2 % for the spiked samples.

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APA: Copy

ABDOLMOHAMMAD ZADEH, HOSSEIN, & HAMMAMI OSKOOYI, SEYED MORTEZA. (2015). SOLID-PHASE EXTRACTION OF L-TRYPTOPHAN FROM FOOD SAMPLES UTILIZING A LAYERED DOUBLE HYDROXIDE NANO-SORBENT PRIOR TO ITS DETERMINATION BY SPECTROFLUOROMETRY. JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS), 12(6), 1115-1122. SID. https://sid.ir/paper/283114/en

Vancouver: Copy

ABDOLMOHAMMAD ZADEH HOSSEIN, HAMMAMI OSKOOYI SEYED MORTEZA. SOLID-PHASE EXTRACTION OF L-TRYPTOPHAN FROM FOOD SAMPLES UTILIZING A LAYERED DOUBLE HYDROXIDE NANO-SORBENT PRIOR TO ITS DETERMINATION BY SPECTROFLUOROMETRY. JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS)[Internet]. 2015;12(6):1115-1122. Available from: https://sid.ir/paper/283114/en

IEEE: Copy

HOSSEIN ABDOLMOHAMMAD ZADEH, and SEYED MORTEZA HAMMAMI OSKOOYI, “SOLID-PHASE EXTRACTION OF L-TRYPTOPHAN FROM FOOD SAMPLES UTILIZING A LAYERED DOUBLE HYDROXIDE NANO-SORBENT PRIOR TO ITS DETERMINATION BY SPECTROFLUOROMETRY,” JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS), vol. 12, no. 6, pp. 1115–1122, 2015, [Online]. Available: https://sid.ir/paper/283114/en

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