DETECTION AND QUANTITATION OF CELLULOLYTIC BACTERIA FIBROBACTER SUCCINOGENES AND RUMINOCOCCUS FLAVEFACIENS IN RUMEN OF HOLSTEIN CALVES, USING QUANTITATIVE PCR TECHNIQUE

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SAREMI B.; NASERIAN A.A.; NASIRI MOHAMMAD REZA; MOHAMMADI AMIR

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Journal: JOURNAL OF AGRICULTURAL KNOWLEDGE Year:2008 | Volume:18 | Issue:1 Page(s): 161-170

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Title

DETECTION AND QUANTITATION OF CELLULOLYTIC BACTERIA FIBROBACTER SUCCINOGENES AND RUMINOCOCCUS FLAVEFACIENS IN RUMEN OF HOLSTEIN CALVES, USING QUANTITATIVE PCR TECHNIQUE

Author(s)

SAREMI B. | NASERIAN A.A. | NASIRI MOHAMMAD REZA | MOHAMMADI AMIR | Issue Writer Certificate

Keywords

CELLULOLYTIC BACTERIAQ3

QUANTITATIVE PCRQ3

RUMENQ3

Abstract

Eighteen female Holstein calves were divided to three groups and received similar basal diet and different levels of yeast (Saccharomyces cerevisiea) daily (0, 0.5 and 1% DM basal diet). RUMEN samples derived at 30, 60 and 90 days old. Results showed that PCR could detect Fibrobacter succinogenes up to two copy of pure DNA. Also it was shown that Fibrobacter succinogenes and Ruminococcus flavefaciens could be detected at RUMEN samples and introduced bands were similar to other studies. In addition multiple-PCR could be used for detection of them. Results showed that QUANTITATIVE PCR could be used for enumeration of Fibrobacter succinogenes and showed that different levels of yeast had no significant effect on population of Fibrobacter succinogenes. Sampling time had a significant effect on population of Fibrobacter succinogenes (P<0.0001) and population of this bacteri in different sampling times (30, 60 and 90 days old) in each treatment increased by time. Generally, it seems that QUANTITATIVE PCR technique could be used for enumeration of Fibrobacter succinogenes in RUMEN samples.

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APA: Copy

SAREMI, B., NASERIAN, A.A., NASIRI, MOHAMMAD REZA, & MOHAMMADI, AMIR. (2008). DETECTION AND QUANTITATION OF CELLULOLYTIC BACTERIA FIBROBACTER SUCCINOGENES AND RUMINOCOCCUS FLAVEFACIENS IN RUMEN OF HOLSTEIN CALVES, USING QUANTITATIVE PCR TECHNIQUE. JOURNAL OF AGRICULTURAL KNOWLEDGE, 18(1), 161-170. SID. https://sid.ir/paper/28643/en

Vancouver: Copy

SAREMI B., NASERIAN A.A., NASIRI MOHAMMAD REZA, MOHAMMADI AMIR. DETECTION AND QUANTITATION OF CELLULOLYTIC BACTERIA FIBROBACTER SUCCINOGENES AND RUMINOCOCCUS FLAVEFACIENS IN RUMEN OF HOLSTEIN CALVES, USING QUANTITATIVE PCR TECHNIQUE. JOURNAL OF AGRICULTURAL KNOWLEDGE[Internet]. 2008;18(1):161-170. Available from: https://sid.ir/paper/28643/en

IEEE: Copy

B. SAREMI, A.A. NASERIAN, MOHAMMAD REZA NASIRI, and AMIR MOHAMMADI, “DETECTION AND QUANTITATION OF CELLULOLYTIC BACTERIA FIBROBACTER SUCCINOGENES AND RUMINOCOCCUS FLAVEFACIENS IN RUMEN OF HOLSTEIN CALVES, USING QUANTITATIVE PCR TECHNIQUE,” JOURNAL OF AGRICULTURAL KNOWLEDGE, vol. 18, no. 1, pp. 161–170, 2008, [Online]. Available: https://sid.ir/paper/28643/en

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