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Information Journal Paper

Title

OPTIMIZATION OF PENICILLIN G ACYLASE PRODUCTION

Pages

  159-165

Keywords

Not Registered.

Abstract

 In this paper, effects of pH, carbon and nitrogen sources, inducer, penicillin G, shaking and, aeration rate on penicillin G acylase (PGA, EC3.5.1.11) synthesis by Escherichia coli were studied and the optimum conditions for batch fermentation process were determined. The maximum activity of the enzyme and the biomass concentration were obtained at pH range of 8.0-9.0, with 250 rpm using 5 g.l-1 yeast extract as both carbon and nitrogen sources and 4 g. g.l-1 of phenyl acetic acid as the inducer. Addition of 0.2 g. g.l-1 penicillin G to the fermentation broth enhanced the enzyme production. The optimum aeration rate in the fermenter was obtained to be 0.85 wm. The growth kinetics of PGA production was analyzed using a second-order model applied to Leudeking-Piret kinetics. The analysis of Leudeking-Piret model demonstrates that PGA production by Escherichia coli obeys the growth-associated kinetics.

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    APA: Copy

    KHEYR ALOLOUMI, A., KAZEMI VEYSARI, A., ARJMAND, M., & DOUSTI, H.. (2001). OPTIMIZATION OF PENICILLIN G ACYLASE PRODUCTION. SCIENTIA IRANICA, 8(3 (BIOTECHNOLOGY AND BIOENIRONMENTAL ENGINEERING)), 159-165. SID. https://sid.ir/paper/289390/en

    Vancouver: Copy

    KHEYR ALOLOUMI A., KAZEMI VEYSARI A., ARJMAND M., DOUSTI H.. OPTIMIZATION OF PENICILLIN G ACYLASE PRODUCTION. SCIENTIA IRANICA[Internet]. 2001;8(3 (BIOTECHNOLOGY AND BIOENIRONMENTAL ENGINEERING)):159-165. Available from: https://sid.ir/paper/289390/en

    IEEE: Copy

    A. KHEYR ALOLOUMI, A. KAZEMI VEYSARI, M. ARJMAND, and H. DOUSTI, “OPTIMIZATION OF PENICILLIN G ACYLASE PRODUCTION,” SCIENTIA IRANICA, vol. 8, no. 3 (BIOTECHNOLOGY AND BIOENIRONMENTAL ENGINEERING), pp. 159–165, 2001, [Online]. Available: https://sid.ir/paper/289390/en

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