PERIPHERAL BLOOD LYMPHOCYTES ARE ABLE TO MAINTAIN THEIR VIABILITY AND BASIC FUNCTION IN NORMAL URINE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AGHAMAJIDI AZIN; BABAIE KHAMENEH HESAM; ABEDIAN ZEINAB; KHORASANI HAMID REZA; MOSTAFAZADEH AMROLLAH

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: AMIRKABIR Year:2016 | Volume:7 | Issue:1 Page(s): 43-47

Download Full-Text

View:

375

Download:

186

Cites:

Information Journal Paper

Title

PERIPHERAL BLOOD LYMPHOCYTES ARE ABLE TO MAINTAIN THEIR VIABILITY AND BASIC FUNCTION IN NORMAL URINE

Author(s)

Keywords

URINE

LYMPHOCYTE LIFESPAN

LYMPHOCYTE ACTIVATION

PHA

Abstract

Background: Similar to inflammatory cells, peripheral blood mononuclear cells (PBMCs) can also infiltrate in to kidney and urinary tracts and subsequently excreted by URINE. In this study we determined the viability rate and response to phytohemagglutinin-A (PHA) of human PBMCs in normal URINE.Methods: A number of 1×106 ficoll-hypaque isolated PBMCs were dispensed in 1 ml normal URINE and 6 molar urea and RPMI-1640+FBS10 % were considered as negative and positive control, respectively. After 20, 60 and 120 minutes the viability of these cells was measured by trypan blue dye exclusion assay. 1×105 of PBMCs were isolated from URINE and cultured as triplicate in RPMI-1640 supplemented with FBS 10% and PHA for 96hr. MTT assay was performed to determine the PBMCs response to PHA. These experiments were repeated three times independently.Results: There was no significant difference between the viability rates of the PBMCs incubated in URINE and positive control after 20, 60 and 120 minutes while after 60 minutes they exhibited 75.6% of reactivity to PHA versus positive control. Overall, there was a significant difference in trends of viability rate across the three groups (p<0.05).Conclusion: Our results showed that not only PBMCs remained alive in URINE after 120 minutes, but can also respond to PHA up to 60 minutes at a remarkable level. These data open a new avenue in the designation for cell culture-based techniques in URINE cell analysis.

Cites

No record.

References

No record.

Cite

APA: Copy

AGHAMAJIDI, AZIN, BABAIE KHAMENEH, HESAM, ABEDIAN, ZEINAB, KHORASANI, HAMID REZA, & MOSTAFAZADEH, AMROLLAH. (2016). PERIPHERAL BLOOD LYMPHOCYTES ARE ABLE TO MAINTAIN THEIR VIABILITY AND BASIC FUNCTION IN NORMAL URINE. AMIRKABIR, 7(1), 43-47. SID. https://sid.ir/paper/323393/en

Vancouver: Copy

AGHAMAJIDI AZIN, BABAIE KHAMENEH HESAM, ABEDIAN ZEINAB, KHORASANI HAMID REZA, MOSTAFAZADEH AMROLLAH. PERIPHERAL BLOOD LYMPHOCYTES ARE ABLE TO MAINTAIN THEIR VIABILITY AND BASIC FUNCTION IN NORMAL URINE. AMIRKABIR[Internet]. 2016;7(1):43-47. Available from: https://sid.ir/paper/323393/en

IEEE: Copy

AZIN AGHAMAJIDI, HESAM BABAIE KHAMENEH, ZEINAB ABEDIAN, HAMID REZA KHORASANI, and AMROLLAH MOSTAFAZADEH, “PERIPHERAL BLOOD LYMPHOCYTES ARE ABLE TO MAINTAIN THEIR VIABILITY AND BASIC FUNCTION IN NORMAL URINE,” AMIRKABIR, vol. 7, no. 1, pp. 43–47, 2016, [Online]. Available: https://sid.ir/paper/323393/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops