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Information Journal Paper

Title

PROTEIN PATTERN OF PENICILLIUM SPECIES ISOLATED FROM NATURAL SOURCES IN IRAN

Pages

  111-115

Abstract

 Objective: Farctionation of different PENICILLIUM species based on PROTEIN bands. Procedure: In this study PENICILLIUM citrinum, PENICILLIUM oxalicum, PENICILLIUM notatum and PENICILLIUM frequents isolated from air in Iran have been compared for their PROTEIN pattern antigens. First, the isolates were cultured on sabouraud dextrose agar medium and then subcultured on czapex agar and were maintained on 30°C for 48-72h. Then they were cultured on sobouraud broth medium for preparing PROTEIN extracts, and braudford method was used for measuring the level of PROTEIN. The PROTEINs were differentiated using SDS-PAGE with 10% separating gel. Coomassie blue G250 was used for staining. Results: 34 PROTEIN band with molecular weight of: 19.5,  24,26,27,28.5,32,36,39,45,48,50,52,53,55,56.5, 59.5,63,65,66.5,68,76,84,88,90,92,93,94,95,97, 107,116,123,128 and 158 kD were observed. The bands 19.5,24,28.5,45,52,53,56.5,59.5,76,84 and 97kD were present in all 30 isolates under study. Clinical implications: The results indicate that there are inter species and intra species differences but there is no significant difference in PROTEIN patterns of the isolates.    

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  • Cite

    APA: Copy

    SABOKBAR, A., KHOSRAVI, A.R., KAYVAN AMINEH, H., MOAZZENI, M., & KAZEMNEJAD, ANOUSHIRAVAN. (2005). PROTEIN PATTERN OF PENICILLIUM SPECIES ISOLATED FROM NATURAL SOURCES IN IRAN. JOURNAL OF VETERINARY RESEARCH, 60(2), 111-115. SID. https://sid.ir/paper/34671/en

    Vancouver: Copy

    SABOKBAR A., KHOSRAVI A.R., KAYVAN AMINEH H., MOAZZENI M., KAZEMNEJAD ANOUSHIRAVAN. PROTEIN PATTERN OF PENICILLIUM SPECIES ISOLATED FROM NATURAL SOURCES IN IRAN. JOURNAL OF VETERINARY RESEARCH[Internet]. 2005;60(2):111-115. Available from: https://sid.ir/paper/34671/en

    IEEE: Copy

    A. SABOKBAR, A.R. KHOSRAVI, H. KAYVAN AMINEH, M. MOAZZENI, and ANOUSHIRAVAN KAZEMNEJAD, “PROTEIN PATTERN OF PENICILLIUM SPECIES ISOLATED FROM NATURAL SOURCES IN IRAN,” JOURNAL OF VETERINARY RESEARCH, vol. 60, no. 2, pp. 111–115, 2005, [Online]. Available: https://sid.ir/paper/34671/en

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