Differentiation of Infectious bursal disease viruses isolated from Iranian poultry flocks using real-time RT-PCR and high resolution melt curve analysis

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Cheraghchibashi m.; PEIGHAMBARI S.M.; HOSSEINI H.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Veterinary Research Year:2017 | Volume:72 | Issue:3 Page(s): 331-339

Download Full-Text

Persian Verion

View:

480

Download:

Cites:

Information Journal Paper

Title

Differentiation of Infectious bursal disease viruses isolated from Iranian poultry flocks using real-time RT-PCR and high resolution melt curve analysis

Author(s)

Cheraghchibashi m. | PEIGHAMBARI S.M. | HOSSEINI H. | Issue Writer Certificate

Keywords

infectious bursal diseaseQ2

high resolution melt curve analysisQ1

real time RT-PCR poultryQ1

Abstract

BACKGROUND: infectious bursal disease (IBD) is a highly contagious disease of young birds. Differentiation between classical virulent and very virulent IBDV (vvIBDV) isolate is very important for the poultry industry to choose the right vaccination program. Molecular and serological tests are time consuming and have variable sensitivity. However, the melting curve analysis is a relatively fast method with high precision. OBJECTIVES: This study was designed to evaluate the efficacy of the melting curve analysis for differentiation of some Iranian IBDVs whose identity had been previously determined by RT-PCR/ RFLP analysis. METHODS: In this study, after RNA extraction and reverse transcription and Real Time RT-PCR of IBDVs, high melting resolution at temperatures ranging from 81 to 92 ° C were performed. RESULTS: The findings of this study showed that in the high resolution melting curve analysis, the viruses were classified from A to D. Three vaccine strains of D78, Gumbokal, Bursa CE; IBD L; Bursine 2; and all field viruses were placed in groups A, B, C, and D, respectively. High resolution melting curve analysis after normalization also showed all viruses of this study were placed in 4 HRM genotypic group. Three strains, D78, Gumbokal, Bursa CE, produced similar and non-differentiable curve but were different from other vaccine and field strains. Two other vaccine strains, IBD L and Bursine 2, were different from each other and other viruses. CONCLUSIONS: We concluded that the real-time RT-PCR HRM technique is cost-effective and reliable among the currently used methods and can be used for differentiation of IBDV isolates.

Cites

No record.

References

No record.

Cite

APA: Copy

Cheraghchibashi, m., PEIGHAMBARI, S.M., & HOSSEINI, H.. (2017). Differentiation of Infectious bursal disease viruses isolated from Iranian poultry flocks using real-time RT-PCR and high resolution melt curve analysis. JOURNAL OF VETERINARY RESEARCH, 72(3 ), 331-339. SID. https://sid.ir/paper/34726/en

Vancouver: Copy

Cheraghchibashi m., PEIGHAMBARI S.M., HOSSEINI H.. Differentiation of Infectious bursal disease viruses isolated from Iranian poultry flocks using real-time RT-PCR and high resolution melt curve analysis. JOURNAL OF VETERINARY RESEARCH[Internet]. 2017;72(3 ):331-339. Available from: https://sid.ir/paper/34726/en

IEEE: Copy

m. Cheraghchibashi, S.M. PEIGHAMBARI, and H. HOSSEINI, “Differentiation of Infectious bursal disease viruses isolated from Iranian poultry flocks using real-time RT-PCR and high resolution melt curve analysis,” JOURNAL OF VETERINARY RESEARCH, vol. 72, no. 3 , pp. 331–339, 2017, [Online]. Available: https://sid.ir/paper/34726/en

Related Journal Papers