EFFECT OF DIFFERENT CONCENTRATIONS OF FETAL CALF SERUM AND ESTROUS SHEEP SERUM ON OVINE OOCYTES MATURATION IN VITRO

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GHASEMZADEH NAVA H.; TAJIK PARVIZ; BOLOURCHI M.

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Journal: Journal of Veterinary Research Year:2000 | Volume:55 | Issue:1 Page(s): 29-32

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Title

EFFECT OF DIFFERENT CONCENTRATIONS OF FETAL CALF SERUM AND ESTROUS SHEEP SERUM ON OVINE OOCYTES MATURATION IN VITRO

Author(s)

GHASEMZADEH NAVA H. | TAJIK PARVIZ | BOLOURCHI M. | Issue Writer Certificate

Keywords

SHEEPQ2

OOCYTE MATURATIONQ3

ESTROUS SHEEP SERUMQ4

FETAL CALF SERUMQ3

Abstract

The aim of this study was to assess the effects of different concentrations of FETAL CALF SERUM and estrous SHEEP serum on in vitro maturation of ovine oocytes. Ovine ovaries were isolated from a local slaughter house and transported to the laboratory. Oocytes were aspirated from the folicles and washed 4 times in TCM-199 with or without different concentrations (10, 15 and 20%) of heat inactivated FETAL CALF SERUM (FCS) or estrous SHEEP serum (ESS), and cultured in TCM-199 under paraffin oil. After 24 h culture for maturation, oocytes were denuded from cumulus cells and were mounted on slide glass, fixed, stained and observed under a phase contrast microscope for evidence of maturation, Very few oocytes were matured in the medium without each serum. However maturation rates were significantly increased (P<0.001) when the media were supplemented with sera. The results of the present study show that the maturation in ESS medium is comparable to FCS.

Cites

POSSIBILITY OF UTILIZATION OF SERUM FROM CATTLE, SHEEP AND MOUSE INSTEAD OF FETAL CALF SERUM (FCS) IN CULTURE OF LEISHMANIA MAJOR

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APA: Copy

GHASEMZADEH NAVA, H., TAJIK, PARVIZ, & BOLOURCHI, M.. (2000). EFFECT OF DIFFERENT CONCENTRATIONS OF FETAL CALF SERUM AND ESTROUS SHEEP SERUM ON OVINE OOCYTES MATURATION IN VITRO. JOURNAL OF VETERINARY RESEARCH, 55(1), 29-32. SID. https://sid.ir/paper/34746/en

Vancouver: Copy

GHASEMZADEH NAVA H., TAJIK PARVIZ, BOLOURCHI M.. EFFECT OF DIFFERENT CONCENTRATIONS OF FETAL CALF SERUM AND ESTROUS SHEEP SERUM ON OVINE OOCYTES MATURATION IN VITRO. JOURNAL OF VETERINARY RESEARCH[Internet]. 2000;55(1):29-32. Available from: https://sid.ir/paper/34746/en

IEEE: Copy

H. GHASEMZADEH NAVA, PARVIZ TAJIK, and M. BOLOURCHI, “EFFECT OF DIFFERENT CONCENTRATIONS OF FETAL CALF SERUM AND ESTROUS SHEEP SERUM ON OVINE OOCYTES MATURATION IN VITRO,” JOURNAL OF VETERINARY RESEARCH, vol. 55, no. 1, pp. 29–32, 2000, [Online]. Available: https://sid.ir/paper/34746/en

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