IN VITRO DEVELOPMENT OF CATTLE EMBRYO AS AFFECTED BY GLUCOSE, SERUM AND EDTA

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PIRESTANI A.; HOSSEINI SEYED MORTEZA; MOULAVI FARIBA; HAJIAN MAHDI; ABEDI PARVANEH; FOROUZANFAR MOHSEN; OSTAD HOSSEINI S.; HOSSEINI LALEH; GHASEMZADEH NAVA H.; TAJIK PARVIZ; NASR ESFAHANI MOHAMMAD HASAN

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Journal: Anatomical Sciences Journal Year:2009 | Volume:7 | Issue:27 Page(s): 11-23

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Title

IN VITRO DEVELOPMENT OF CATTLE EMBRYO AS AFFECTED BY GLUCOSE, SERUM AND EDTA

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Keywords

IN VITRO EMBRYO DEVELOPMENTQ3

BOVINE ZYGOTESQ3

SOFQ3

SERUMQ3

Abstract

Purpose: The aim of this study was to evaluate the effect of different modifications of sequential synthetic oviductal_fluid (SOF) culture system on developmental competence of in vitro matured/fertilized cattle embryos.Materials and Methods: Bovine oocytes were matured and fertilized in vitro and then presumptive zygotes were randomly cultured for up to 9 days in different modifications of SOF culture system to consider the effects of glucose, SERUM and EDTA on embryo development.Results: All the embryo culture systems were efficient to support bovine embryo development till blastocyst stage. There was no significant difference in the ratios of embryos; however, the ratios of blastocyst and also hatchability of embryos cultured in SOF C (51.3%, 43.0% and 83.8%, respectively) were significantly higher than those of all the other SOF groups. Furthermore, while glucose had a partial improving effect on embryo development, a significant decrease in embryo development beyond the morula stage was observed in embryos cultured in SOF system with initial supplementation of EDTA compared with all the other groups. Conclusion: It was concluded that appropriate modifications of SOF culture systems can result insignificantly great IN VITRO EMBRYO DEVELOPMENT.

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APA: Copy

PIRESTANI, A., HOSSEINI, SEYED MORTEZA, MOULAVI, FARIBA, HAJIAN, MAHDI, ABEDI, PARVANEH, FOROUZANFAR, MOHSEN, OSTAD HOSSEINI, S., HOSSEINI, LALEH, GHASEMZADEH NAVA, H., TAJIK, PARVIZ, & NASR ESFAHANI, MOHAMMAD HASAN. (2009). IN VITRO DEVELOPMENT OF CATTLE EMBRYO AS AFFECTED BY GLUCOSE, SERUM AND EDTA. JOURNAL OF IRANIAN ANATOMICAL SCIENCES, 7(27), 11-23. SID. https://sid.ir/paper/81551/en

Vancouver: Copy

PIRESTANI A., HOSSEINI SEYED MORTEZA, MOULAVI FARIBA, HAJIAN MAHDI, ABEDI PARVANEH, FOROUZANFAR MOHSEN, OSTAD HOSSEINI S., HOSSEINI LALEH, GHASEMZADEH NAVA H., TAJIK PARVIZ, NASR ESFAHANI MOHAMMAD HASAN. IN VITRO DEVELOPMENT OF CATTLE EMBRYO AS AFFECTED BY GLUCOSE, SERUM AND EDTA. JOURNAL OF IRANIAN ANATOMICAL SCIENCES[Internet]. 2009;7(27):11-23. Available from: https://sid.ir/paper/81551/en

IEEE: Copy

A. PIRESTANI, SEYED MORTEZA HOSSEINI, FARIBA MOULAVI, MAHDI HAJIAN, PARVANEH ABEDI, MOHSEN FOROUZANFAR, S. OSTAD HOSSEINI, LALEH HOSSEINI, H. GHASEMZADEH NAVA, PARVIZ TAJIK, and MOHAMMAD HASAN NASR ESFAHANI, “IN VITRO DEVELOPMENT OF CATTLE EMBRYO AS AFFECTED BY GLUCOSE, SERUM AND EDTA,” JOURNAL OF IRANIAN ANATOMICAL SCIENCES, vol. 7, no. 27, pp. 11–23, 2009, [Online]. Available: https://sid.ir/paper/81551/en

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