STUDY ON PROTEINAL PATTERN OF ISOLATED NDV IN IRAN AND COMPARISON WITH LIVE VACCIN STRAINS

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HEMATZADEH FARHID; ALI NEZHAD AREZOU

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Journal of Veterinary Research Year:2003 | Volume:58 | Issue:1 Page(s): 61-65

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Information Journal Paper

Title

STUDY ON PROTEINAL PATTERN OF ISOLATED NDV IN IRAN AND COMPARISON WITH LIVE VACCIN STRAINS

Author(s)

HEMATZADEH FARHID | ALI NEZHAD AREZOU | Issue Writer Certificate

Keywords

NEWCASTLE DISEASE VIRUSQ2

ELECTROPHORESISQ3

POLYACRYLAMIDE GELLQ4

81 VACCINQ4

LASOTA VACCINQ3

PROTEINQ4

Abstract

Objective: In this research, PROTEIN patterns of 30 isolates of NEWCASTLE DISEASE VIRUS (isolated in Iran) along with two vaccinal strains (81 and Lasota) were determined.Design: Descriptive study.Samples: These viruses were isolated from Jun.2000 to Oet.2001 out of 507 brains of chicken suspected to infection with virulent NDY. Procedure: Following preparation and inoculation of 507 brain of infected chicken in allantoic cavity of 8 days embryonated eggs, 30 isolates of virus that identified by HI test, were isolated. These samples along with 2 vaccinal strains, 8 I and Lasota were purified by high-speed centrifugation and then ELECTROPHORESIS by SDSPAGE method.Results: The results of SDS-PAGE test of samples, showed that the isolated viruses posses 80, 72, 64, 43, 27 and 23 kDa bands in their PROTEIN patterns. No difference in virulent and a virulent virulent (81 and Lasota) strains in PROTEIN patterns was observed. Only in three cases of virulent strains of NDV, 48-49 kDa bands were observed instead of 64 kDa band. This PROTEIN has not been previously identified.

Cites

DESIGNING OF A SPECIFIC IMMUNOFLUORESCENCE METHOD FOR DIFFERENTIATION OF VELOGENIC AND VACCINAL STRAINS OF NEWCASTLE DISEASE VIRUS

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APA: Copy

HEMATZADEH, FARHID, & ALI NEZHAD, AREZOU. (2003). STUDY ON PROTEINAL PATTERN OF ISOLATED NDV IN IRAN AND COMPARISON WITH LIVE VACCIN STRAINS. JOURNAL OF VETERINARY RESEARCH, 58(1), 61-65. SID. https://sid.ir/paper/35079/en

Vancouver: Copy

HEMATZADEH FARHID, ALI NEZHAD AREZOU. STUDY ON PROTEINAL PATTERN OF ISOLATED NDV IN IRAN AND COMPARISON WITH LIVE VACCIN STRAINS. JOURNAL OF VETERINARY RESEARCH[Internet]. 2003;58(1):61-65. Available from: https://sid.ir/paper/35079/en

IEEE: Copy

FARHID HEMATZADEH, and AREZOU ALI NEZHAD, “STUDY ON PROTEINAL PATTERN OF ISOLATED NDV IN IRAN AND COMPARISON WITH LIVE VACCIN STRAINS,” JOURNAL OF VETERINARY RESEARCH, vol. 58, no. 1, pp. 61–65, 2003, [Online]. Available: https://sid.ir/paper/35079/en

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