Trace Determination of Duloxetine in Human Plasma by a Novel Ionic Liquid-Based Ultrasound-Assisted In Situ Solvent Formation Microextraction and High-Performance Liquid Chromatography

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ZEEB MOHSEN; FARAHANI HADI

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Journal Paper

Paper Information

Journal: ANALYTICAL AND BIOANALYTICAL CHEMISTRY RESEARCH Year:2018 | Volume:5 | Issue:2 Page(s): 183-193

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Title

Trace Determination of Duloxetine in Human Plasma by a Novel Ionic Liquid-Based Ultrasound-Assisted In Situ Solvent Formation Microextraction and High-Performance Liquid Chromatography

Author(s)

ZEEB MOHSEN | FARAHANI HADI | Issue Writer Certificate

Keywords

Ionic liquid-Based ultrasound-assisted in situ solvent formation microextraction

Human plasma

High-performance liquid chromatography

Duloxetine

Abstract

For the first time, a novel and efficient Ionic liquid-Based ultrasound-assisted in situ solvent formation microextraction (IL-UA-ISFME) combined with High-performance liquid chromatography-ultraviolet detection (HPLC-UV) has been successfully developed for the determination of Duloxetine (DLX) in Human plasma. Herein, an environmentally-friendly hydrophobic ionic liquid (1-butyl-3-methylimidazolium hexafluorophosphate) was formed by addition of a hydrophilic ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate) to sample solution including NaPF6 as an ion-pairing agent. The analyte was extracted into the ionic liquid although the microextraction solvent was dispersed among the sample solution using ultrasound radiation. The sample was then centrifuged and extracting phase injected into HPLC system. The developed sample enrichment method revealed a considerable robustness against the variations of sample ionic strength. Parameters controlling the performance of the microextraction were evaluated and optimized. The limit of detection was 0. 8 μ g l-1 while a good linearity (r2 = 0. 996) and a broad linear range (2. 0-1500 μ g l-1) were achieved. A reasonable relative recoveries (83. 6-92. 1%) and the appropriate intra-assay (4. 0-5. 1%, n = 5) and inter-assay (4. 3-7. 6%, n = 9) precisions along with an appropriate sample clean-up exhibited good performance of the analytical procedure. It was eventually validated for the screening purposes in Human plasma after oral administration of the drug and some pharmacokinetic data were achieved. This green method is prompt, convenient, and reliable and offers satisfactory reproducibility as well as sufficient sensitivity.

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APA: Copy

ZEEB, MOHSEN, & FARAHANI, HADI. (2018). Trace Determination of Duloxetine in Human Plasma by a Novel Ionic Liquid-Based Ultrasound-Assisted In Situ Solvent Formation Microextraction and High-Performance Liquid Chromatography. ANALYTICAL AND BIOANALYTICAL CHEMISTRY RESEARCH, 5(2), 183-193. SID. https://sid.ir/paper/354371/en

Vancouver: Copy

ZEEB MOHSEN, FARAHANI HADI. Trace Determination of Duloxetine in Human Plasma by a Novel Ionic Liquid-Based Ultrasound-Assisted In Situ Solvent Formation Microextraction and High-Performance Liquid Chromatography. ANALYTICAL AND BIOANALYTICAL CHEMISTRY RESEARCH[Internet]. 2018;5(2):183-193. Available from: https://sid.ir/paper/354371/en

IEEE: Copy

MOHSEN ZEEB, and HADI FARAHANI, “Trace Determination of Duloxetine in Human Plasma by a Novel Ionic Liquid-Based Ultrasound-Assisted In Situ Solvent Formation Microextraction and High-Performance Liquid Chromatography,” ANALYTICAL AND BIOANALYTICAL CHEMISTRY RESEARCH, vol. 5, no. 2, pp. 183–193, 2018, [Online]. Available: https://sid.ir/paper/354371/en

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