Comparison of Two Brucella abortus and Brucella melitensis Antigens Used in Ewe’ s MRT

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MAKTABI S.; ZAREI M.; GHORBANPOUR M.; Tahmasebi t.; PAKNEJAD M.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Veterinary Research Year:2018 | Volume:73 | Issue:3 Page(s): 291-297

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,623

Download:

Cites:

Information Journal Paper

Title

Comparison of Two Brucella abortus and Brucella melitensis Antigens Used in Ewe’ s MRT

Author(s)

Keywords

Brucella abortusQ2

Brucella melitensisQ2

EweQ1

MRTQ2

PCRQ1

Abstract

BACKGROUND: Brucellosis is one of the most dangerous worldwide infectious zoonotic diseases that are common between ruminants and human. Consumption of infected milk and by-products is the major transmission source to human. In Iran, sheep compared to cow, has a higher rate of contamination with brucellosis. Therefore, early detection and precision could be a starting point for any efficient program to control the disease in human and animals. For brucellosis monitoring, milk ring test (MRT) is recommended but the test is not reliable in sheep herds. Perhaps a more realistic outcome could be achieved by changing the antigen used in MRT. OBJECTIVES: Comparison of two Brucella abortus and Brucella melitensis antigens in MRT for detection of Brucella antibodies in milk, as well as monitoring contamination of Ewe’ s milk in Dezful region by detection of B. abortus and B. melitensis genes using PCR. METHODS: In this research, 220 milk samples from 16 different herds were collected from Dezful region’ s nomadic at Khuzestan province. As the first step, MRT by two antigens, B. abortus and B. melitensis, were conducted on the samples. Next, the samples were subjected to detect Brucella genes using PCR technique. RESULTS: Results showed that 47 (21/3 %) out of 220 cases were positive by MRT test, in terms of both antigens of B. abortus and B. melitensis. In PCR, out of 220 samples, only 9 (4%) samples were positive for specific genes of B. melitensis which were MRT positive as well. CONCLUSIONS: A significant difference between B. abortus and B. melitensis antigens was not observed in MRT. Although the nature and basis of PCR and MRT methods for the diagnosis of brucellosis is different but a significant difference between the results obtained by PCR and MRT showed that MRT even by changing of antigens is still not authentic. Considering that various methods of identification have their limitations, it is recommended that in Ewe’ s milk samples, in addition to using a serological method as screening, PCR and culture methods should be used for definitive diagnosis.

Cites

No record.

References

No record.

Cite

APA: Copy

MAKTABI, S., ZAREI, M., GHORBANPOUR, M., Tahmasebi, t., & PAKNEJAD, M.. (2018). Comparison of Two Brucella abortus and Brucella melitensis Antigens Used in Ewe’ s MRT. JOURNAL OF VETERINARY RESEARCH, 73(3 ), 291-297. SID. https://sid.ir/paper/35564/en

Vancouver: Copy

MAKTABI S., ZAREI M., GHORBANPOUR M., Tahmasebi t., PAKNEJAD M.. Comparison of Two Brucella abortus and Brucella melitensis Antigens Used in Ewe’ s MRT. JOURNAL OF VETERINARY RESEARCH[Internet]. 2018;73(3 ):291-297. Available from: https://sid.ir/paper/35564/en

IEEE: Copy

S. MAKTABI, M. ZAREI, M. GHORBANPOUR, t. Tahmasebi, and M. PAKNEJAD, “Comparison of Two Brucella abortus and Brucella melitensis Antigens Used in Ewe’ s MRT,” JOURNAL OF VETERINARY RESEARCH, vol. 73, no. 3 , pp. 291–297, 2018, [Online]. Available: https://sid.ir/paper/35564/en

Related Journal Papers