DETECTION OF BRUCELLA ABORTUS BY PCR ASSAY AND COMPARISON WITH CULTURE ASSAY

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HOSSEINIDOUST S.R.; AHMADI A.; AHMADI Z.; HAJIA M.; SAFIRI Z.; GOLMANESH L.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Military Medicine Year:2005 | Volume:7 | Issue:3 Page(s): 239-244

Download Full-Text

Persian Verion

View:

3,603

Download:

Cites:

Information Journal Paper

Title

DETECTION OF BRUCELLA ABORTUS BY PCR ASSAY AND COMPARISON WITH CULTURE ASSAY

Author(s)

Keywords

BRUCELLA ABORTUSQ4

PCRQ3

CULTURE METHODQ4

DETECTIONQ3

Abstract

Introduction: Brucellosis is a zoonotic disease with global prevalence. It has been prevalent in different parts of Iran, since previous decades. The control of brucellosis is urgent from medical and economic point of view. Accurate diagnosis of the agent of disease in clinical samples has been highlighted as the first step of disease control. Different culture and serologic methods have the vital role in diagnosis of brucellosis although they have shown some practical difficulties as well. In the present investigation, the species specific primer set for B. abortus were used and PCR protocol were evaluated with clinical and veterinary samples. Material and Method: Standard B. abortus were obtained from national reference laboratory and kept under laboratory conditions. All primer set specific for different targets of bacteria were studied and evaluated by computer software. The genomic fragment of IS711 were selected for the study. The genomic extraction were then achieved and the primer set were optimized for the laboratory conditions. All clinical (human & veterinary) isolates were then tested with optimized primer set and the results were compared with other data sets. Results: Among 42 serologic positive samples, the bacteria were isolated from only six samples. 10 animal samples were examined with both culture and PCR methods. The sensitivity of PCR was 60% while the sensitivity of culture was 40%. Meanwhile, the PCR sensitivity for 5 human samples (80%) were less than CULTURE METHOD (100%). Discussion: Serologic results for clinical samples were compared with culture results and also with PCR. Although the PCR set were optimized in our laboratory the results of PCR were not in accordance with CULTURE METHOD. All clinical samples were mentioned as positive according to serologic results while brucella spp. were not isolated from all samples. We could expect more sensitivity from PCR set, but one culture positive sample was negative with PCR. This could be due to specific gene target of B. abortus even not all strains of B. abortus were practically detectable with tested primer sets. The future work could be focused on multiplex PCR for all strains of B. abortus.

Cites

MOLECULAR DIAGNOSIS OF BRUCELLOSIS-CAUSING BACTERIA ISOLATED FROM PATIENTS WITH BRUCELLOSIS WITH THE HELP OF TRPE AND OMP25 GENES BY PCR

References

Cite

APA: Copy

HOSSEINIDOUST, S.R., AHMADI, A., AHMADI, Z., HAJIA, M., SAFIRI, Z., & GOLMANESH, L.. (2005). DETECTION OF BRUCELLA ABORTUS BY PCR ASSAY AND COMPARISON WITH CULTURE ASSAY. JOURNAL OF MILITARY MEDICINE, 7(3), 239-244. SID. https://sid.ir/paper/62173/en

Vancouver: Copy

HOSSEINIDOUST S.R., AHMADI A., AHMADI Z., HAJIA M., SAFIRI Z., GOLMANESH L.. DETECTION OF BRUCELLA ABORTUS BY PCR ASSAY AND COMPARISON WITH CULTURE ASSAY. JOURNAL OF MILITARY MEDICINE[Internet]. 2005;7(3):239-244. Available from: https://sid.ir/paper/62173/en

IEEE: Copy

S.R. HOSSEINIDOUST, A. AHMADI, Z. AHMADI, M. HAJIA, Z. SAFIRI, and L. GOLMANESH, “DETECTION OF BRUCELLA ABORTUS BY PCR ASSAY AND COMPARISON WITH CULTURE ASSAY,” JOURNAL OF MILITARY MEDICINE, vol. 7, no. 3, pp. 239–244, 2005, [Online]. Available: https://sid.ir/paper/62173/en

Related Journal Papers