Effect of plant growth regulators on Fittonia verschaffeltii regeneration at in vitro conditions

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Rashidi Mahsa; Saremi rad Ali; Froozesh Peyman; Ghasemo Omran Vali Ollah

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Journal: JOURNAL OF PLANT PHYSIOLOGY AND BREEDING Year:2018 | Volume:8 | Issue:2 Page(s): 59-68

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Title

Effect of plant growth regulators on Fittonia verschaffeltii regeneration at in vitro conditions

Author(s)

Rashidi Mahsa | Saremi rad Ali | Froozesh Peyman | Ghasemo Omran Vali Ollah | Issue Writer Certificate

Keywords

Callus inductionQ1

ExplantQ2

Fittonia verschaffeltiiQ1

In vitro cultureQ1

Plant growth regulatorQ1

Abstract

We investigated in vitro regeneration ability of Fittonia verschaffeltii using different Explants (leaf, node) and Plant growth regulators ((2, 4-D, indoleacetic acid, indolebutyric acid (IBA), 6-benzylaminopurine (BAP), thidiazuron)). Percentage of Callus induction, number of shoots, shoot length, percentage of rooting and root length were measured. The leaf Explant showed higher frequency of Callus induction in comparison with the node Explant, while node had higher frequency of shoot formation. There were significant differences among Plant growth regulators in terms of percentage of Callus induction, number of shoots, percentage of rooting and root length, however, the difference was dependent on the type of Explant. The highest percentage of Callus induction (86. 12%) was obtained for the leaf Explant at Murashige and Skoog (MS) medium supplemented with 1 mg/L 2, 4-D and 1 mg/L IBA. The highest number of shoots (5. 9 per Explant) and shoot length (7. 95 cm) were observed for the node Explant at MS medium supplemented with 2 mg/L BAP + 1 mg/L IBA and MS medium containing 1mg/L BAP + 1 mg/L IBA, respectively. The highest percentage of root formation (87%) and root length (10. 1 cm) were obtained at MS medium supplemented with 0. 2 mg/L IBA and MS medium with no hormones, respectively.

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APA: Copy

Rashidi, Mahsa, Saremi rad, Ali, Froozesh, Peyman, & Ghasemo Omran, Vali Ollah. (2018). Effect of plant growth regulators on Fittonia verschaffeltii regeneration at in vitro conditions. JOURNAL OF PLANT PHYSIOLOGY AND BREEDING, 8(2 ), 59-68. SID. https://sid.ir/paper/358129/en

Vancouver: Copy

Rashidi Mahsa, Saremi rad Ali, Froozesh Peyman, Ghasemo Omran Vali Ollah. Effect of plant growth regulators on Fittonia verschaffeltii regeneration at in vitro conditions. JOURNAL OF PLANT PHYSIOLOGY AND BREEDING[Internet]. 2018;8(2 ):59-68. Available from: https://sid.ir/paper/358129/en

IEEE: Copy

Mahsa Rashidi, Ali Saremi rad, Peyman Froozesh, and Vali Ollah Ghasemo Omran, “Effect of plant growth regulators on Fittonia verschaffeltii regeneration at in vitro conditions,” JOURNAL OF PLANT PHYSIOLOGY AND BREEDING, vol. 8, no. 2 , pp. 59–68, 2018, [Online]. Available: https://sid.ir/paper/358129/en

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