Evaluation of the effect of iron sulfate on growth and some biochemical parameters of garlic plantlets under in vitro culture condition

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FATHI REZAEI P.; MOHAMMADNEZHAD M.; AGHAEE A.

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Journal: Journal of Plant Research Year:2020 | Volume:32 | Issue:4 Page(s): 00-00

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Title

Evaluation of the effect of iron sulfate on growth and some biochemical parameters of garlic plantlets under in vitro culture condition

Author(s)

FATHI REZAEI P. | MOHAMMADNEZHAD M. | AGHAEE A. | Issue Writer Certificate

Keywords

AllicinQ1

cysteineQ2

fresh weightQ2

garlicQ2

proteinQ2

Abstract

Allicin as the best-known active compound of garlic (Allium sativum) has a vast variety of biological effects. The effect of iron sulfate was evaluated on Allicin, cysteine, growth and protein contents of Allium sativum explants. garlic bulbs were surfacesterilized and cultured on Murashige and Skoog (MS) medium for 1 month then transferred to the medium supplemented with different concentrations of iron sulfate for 10 and 20 day periods. At the end, shoot and root samples were gathered and fresh weighted and length of plantlets measured. Allicin content was determined by HPLC method, cysteine and total protein contents were determined by spectrophotometry. The highest amount of Allicin and protein was observed for 11. 12 mg/L treatment of root and shoot at both endpoints. The maximum content of Allicin and protein were determined respectively on the root (16. 62 mM/g FW) and shoot (8 mg/g FW) of 11. 12 mg/Ltreated explants after 20 days. On 5. 56 mg/L-treated explants, a significant increase of fresh weight and length of root and cysteine content were observed in comparison with other treatments. In addition, on the shoot of 1. 39 mg/L-treated explants, a significant difference in fresh weight and length were measured compared with the other treatments. Because of the important role of Allicin on medicinal features of Allium sativum, probably iron sulfate could be a good elicitor for the elevation of Allicin content of garlic explants.

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APA: Copy

FATHI REZAEI, P., MOHAMMADNEZHAD, M., & AGHAEE, A.. (2020). Evaluation of the effect of iron sulfate on growth and some biochemical parameters of garlic plantlets under in vitro culture condition. JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 32(4 ), 00-00. SID. https://sid.ir/paper/360362/en

Vancouver: Copy

FATHI REZAEI P., MOHAMMADNEZHAD M., AGHAEE A.. Evaluation of the effect of iron sulfate on growth and some biochemical parameters of garlic plantlets under in vitro culture condition. JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2020;32(4 ):00-00. Available from: https://sid.ir/paper/360362/en

IEEE: Copy

P. FATHI REZAEI, M. MOHAMMADNEZHAD, and A. AGHAEE, “Evaluation of the effect of iron sulfate on growth and some biochemical parameters of garlic plantlets under in vitro culture condition,” JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 32, no. 4 , pp. 00–00, 2020, [Online]. Available: https://sid.ir/paper/360362/en

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