Phylogenetic analysis of cytochrome oxidase subunit 1 from the Mesobuthus eupeus (Scorpions: Buthidae) of Khuzestan province

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Nikkhah N.; JOLODAR A.; TAGHAVI MOGHADAM A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL Year:2018 | Volume:14 | Issue:3 Page(s): 102-111

Download Full-Text

Persian Verion

View:

417

Download:

Cites:

Information Journal Paper

Title

Phylogenetic analysis of cytochrome oxidase subunit 1 from the Mesobuthus eupeus (Scorpions: Buthidae) of Khuzestan province

Author(s)

Nikkhah N. | JOLODAR A. | TAGHAVI MOGHADAM A. | Issue Writer Certificate

Keywords

Mesobuthus eupeusQ2

ScorpionQ1

PhylogeneticQ2

Cytochrome oxidaseQ1

KhuzestanQ2

Abstract

Ten Scorpion samples Mesobuthus eupeus were collected from Baghmalek region in the Khuzestan province of Iran before were identified by Razi Vaccine and Serum Research Institute reference laboratory of Ahvaz. Then, DNA was extracted by phenol/chloroform method in the Laboratory of Molecular Biology in the Faculty of Veterinary Medicine of Shahid Chamran University of Ahvaz. The molecular Phylogenetic analysis of Mesobuthus eupeus is carried out based on sequence data of 623 nucleotides fragment of cytochrome C oxidase subunit I. The gene fragments were amplified by PCR using the specific forward and reverse primers. PCR products were fractionated by agarose gel electrophoresis prior to purifying using gel extraction kit. The purified DNA was sequenced by an Applied Biosystems DNA sequencer via Gene Fanavaran Company. In order to confirm the sequencing data, each gene fragment was sequenced in both directions. In order to compare the sequence data with the similar sequences from other Scorpions, the target sequence data from different Scorpions were retrieved from the Genbank using nblast program via NCBI website. Multiple alignments of the deduced amino acid sequence of cytochrome C oxidase subunit I exhibited 92 and 91% identity to the homologous M. martensii and M. gibossos, respectively. The highest level of identity was scored with M. eupeus philipsi (93%). The results of Phylogenetic analysis using Cytochrome oxidase subunit 1 indicate that the sequence data of Khuzestan Scorpion Mesobuthus eupeus is slightly different from M. eupeusphilipsi gene. As regards of this discrepancy, it concluded that these two Mesobuthus species with highly similar morphological features possibly belonging to two different subspecies.

Cites

No record.

References

No record.

Cite

APA: Copy

Nikkhah, N., JOLODAR, A., & TAGHAVI MOGHADAM, A.. (2018). Phylogenetic analysis of cytochrome oxidase subunit 1 from the Mesobuthus eupeus (Scorpions: Buthidae) of Khuzestan province. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, 14(3 ), 102-111. SID. https://sid.ir/paper/361642/en

Vancouver: Copy

Nikkhah N., JOLODAR A., TAGHAVI MOGHADAM A.. Phylogenetic analysis of cytochrome oxidase subunit 1 from the Mesobuthus eupeus (Scorpions: Buthidae) of Khuzestan province. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL[Internet]. 2018;14(3 ):102-111. Available from: https://sid.ir/paper/361642/en

IEEE: Copy

N. Nikkhah, A. JOLODAR, and A. TAGHAVI MOGHADAM, “Phylogenetic analysis of cytochrome oxidase subunit 1 from the Mesobuthus eupeus (Scorpions: Buthidae) of Khuzestan province,” SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, vol. 14, no. 3 , pp. 102–111, 2018, [Online]. Available: https://sid.ir/paper/361642/en

Related Journal Papers