Determination of suitable housekeeping genes for normalization of quantitative real time PCR analysis of Avicennia marina under crude oil treatment

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Moradi babak; ZARE MAIVAN HASSAN; Sorahinobar Mona; SEYED HASHTROUDI MEHRI

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Journal: JOURNAL OF MARINE SCIENCES AND TECHNOLOGY Year:2019 | Volume:17 | Issue:4 Page(s): 58-69

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Title

Determination of suitable housekeeping genes for normalization of quantitative real time PCR analysis of Avicennia marina under crude oil treatment

Author(s)

Moradi babak | ZARE MAIVAN HASSAN | Sorahinobar Mona | SEYED HASHTROUDI MEHRI | Issue Writer Certificate

Keywords

Mangrove plantsQ1

Avicennia marinaQ1

Quantitative real-time PCRQ1

Reference geneQ1

NormalizationQ2

Oil contaminationQ2

Abstract

Gene expression studies could provide insight into the physiological mechanisms and strategies used by plants under stress conditions. Selection of suitable internal control gene(s) is essential to accurately assess gene expression levels. For the mangrove plant, Avicennia marina, reliable Reference genes to normalize real-time quantitative PCR data has not been previously investigated. In this study, the expression stabilities of four candidate Reference genes [Actin 2 (ACT2), Serine/threonine-protein phosphatase 2A subunit A3 (PP2AA3), TIP-Like (TIP), polyubiquitin 10 (UBQ)] were determined in leaves and roots of A. marina treated by different levels of oils contamination. Three software programs (Bestkeeper, NormFinder and geNorm) were employed to analyze and rank the tested genes. Results showed that ACT2 was the most suitable Reference gene in A. marina and the combination of two or three genes was recommended for greater accuracy. Identification of A. marina Reference genes in a wide range of experimental samples will provide a useful reference in future gene expression studies in this species, particularly involving similar stresses.

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APA: Copy

Moradi, babak, ZARE MAIVAN, HASSAN, Sorahinobar, Mona, & SEYED HASHTROUDI, MEHRI. (2019). Determination of suitable housekeeping genes for normalization of quantitative real time PCR analysis of Avicennia marina under crude oil treatment. JOURNAL OF MARINE SCIENCES AND TECHNOLOGY, 17(4 ), 58-69. SID. https://sid.ir/paper/363050/en

Vancouver: Copy

Moradi babak, ZARE MAIVAN HASSAN, Sorahinobar Mona, SEYED HASHTROUDI MEHRI. Determination of suitable housekeeping genes for normalization of quantitative real time PCR analysis of Avicennia marina under crude oil treatment. JOURNAL OF MARINE SCIENCES AND TECHNOLOGY[Internet]. 2019;17(4 ):58-69. Available from: https://sid.ir/paper/363050/en

IEEE: Copy

babak Moradi, HASSAN ZARE MAIVAN, Mona Sorahinobar, and MEHRI SEYED HASHTROUDI, “Determination of suitable housekeeping genes for normalization of quantitative real time PCR analysis of Avicennia marina under crude oil treatment,” JOURNAL OF MARINE SCIENCES AND TECHNOLOGY, vol. 17, no. 4 , pp. 58–69, 2019, [Online]. Available: https://sid.ir/paper/363050/en

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