IDENTIFICATION AND CHARACTERIZATION OF GLUTATHIONE S-TRANSFERASE CLASS MU FROM THE RUTILUS FRISII KUTUM

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AHMADI SALMAN; Ghafouri Hossein; SARIKHAN SAJJAD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY Year:2018 | Volume:6 | Issue:1 Page(s): 65-78

Download Full-Text

Persian Verion

View:

355

Download:

Cites:

Information Journal Paper

Title

IDENTIFICATION AND CHARACTERIZATION OF GLUTATHIONE S-TRANSFERASE CLASS MU FROM THE RUTILUS FRISII KUTUM

Author(s)

AHMADI SALMAN | Ghafouri Hossein | SARIKHAN SAJJAD | Issue Writer Certificate

Keywords

GLUTATHIONE S-TRANSFERASEQ1

MU CLASSQ1

CASPIAN SEAQ2

RUTILUS FRISII KUTUMQ2

Abstract

GLUTATHIONE S-TRANSFERASEs are one of the key enzymes in phase II of drug metabolism which play role in detoxification and oxidative stress metabolism in prokaryote and eukaryote cells, by binding reduced glutathione to the xenobiotics. Considering the importance of these enzymes in defying against pollution and in regard to the increasing amount of pollution in the CASPIAN SEA during recent years, a deeper knowledge about role and function of this enzyme as a key part of detoxification against the pollutants seems crucial. The goal of this study is identification and gene sequencing of GLUTATHIONE S-TRANSFERASE Mu in RUTILUS FRISII KUTUM. After RNA extraction from male fishes’ liver tissue, cDNA synthesis was the next step followed by PCR. Then the desired gene was inserted into vector pTZ57R/T followed by chemical transformation into E. coli DH5α competent cells. After plasmid extraction and gene sequencing, the partial GSTM sequence with 625 nucleotides was obtained. The nucleotide BLAST result shows more than 89% of identity with GSTM in other members of Cyprinidae that N-terminal region has a greater portion of this identity compared with C-terminal. The most abundant residue found in the GSTM sequence was lysine which has an important role in GLUTATHIONE S-TRANSFERASE structures. The GSTM nucleotide sequence was registered at the NCBI database with accession number KY211992.

Cites

No record.

References

No record.

Cite

APA: Copy

AHMADI, SALMAN, Ghafouri, Hossein, & SARIKHAN, SAJJAD. (2018). IDENTIFICATION AND CHARACTERIZATION OF GLUTATHIONE S-TRANSFERASE CLASS MU FROM THE RUTILUS FRISII KUTUM. AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY, 6(1 ), 65-78. SID. https://sid.ir/paper/363168/en

Vancouver: Copy

AHMADI SALMAN, Ghafouri Hossein, SARIKHAN SAJJAD. IDENTIFICATION AND CHARACTERIZATION OF GLUTATHIONE S-TRANSFERASE CLASS MU FROM THE RUTILUS FRISII KUTUM. AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY[Internet]. 2018;6(1 ):65-78. Available from: https://sid.ir/paper/363168/en

IEEE: Copy

SALMAN AHMADI, Hossein Ghafouri, and SAJJAD SARIKHAN, “IDENTIFICATION AND CHARACTERIZATION OF GLUTATHIONE S-TRANSFERASE CLASS MU FROM THE RUTILUS FRISII KUTUM,” AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY, vol. 6, no. 1 , pp. 65–78, 2018, [Online]. Available: https://sid.ir/paper/363168/en

Related Journal Papers