INVESTIGATION OF SUCROSE EFFECT ON TROPANE ALKALOID PRODUCTION AND SEVERAL BIOCHEMICAL PARAMETERS OF DATURA STRAMONIUM UNDER IN VITRO CULTURE CONDITION

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FATHI REZAEI P.; RAKEE E.

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Journal: Journal of Molecular and Cellular Researches Year:2017 | Volume:30 | Issue:4 Page(s): 558-571

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Title

INVESTIGATION OF SUCROSE EFFECT ON TROPANE ALKALOID PRODUCTION AND SEVERAL BIOCHEMICAL PARAMETERS OF DATURA STRAMONIUM UNDER IN VITRO CULTURE CONDITION

Author(s)

FATHI REZAEI P. | RAKEE E. | Issue Writer Certificate

Keywords

CATALASEQ2

D. STRAMONIUMQ2

GUAIACOL PEROXIDASEQ1

HYOSCYAMINEQ1

SCOPOLAMINEQ1

Abstract

Datura stramoniumas a well-known medicinal plant is rich in tropane alkaloids such as HYOSCYAMINE and SCOPOLAMINE and currently the vast majority of the studies have been focused on optimization of production conditions of these valuable materials in the world. In this research, the effect of sucrose (15, 30 and 45 g/L) as an abiotic elicitor was investigated on fresh weight (FW), HYOSCYAMINE and SCOPOLAMINE production, protein content, activity of CATALASE (CAT) and GUAIACOL PEROXIDASE (GPX) ofD.stramonium. The amount of hyoscumine and SCOPOLAMINE were measured by high-performance liquid chromatography (HPLC) and spectrophotometery method was applied for studying of total protrin content and activity of CAT and GPX. According to the results, the highest amount of fresh weight (0.82 g), production of HYOSCYAMINE (5 mg/g FW), SCOPOLAMINE (20 mg/g FW) and CAT activity (0.5 U/mg protein) were recorded on the root of 45 g/L-treated plantlets but the highest amount of protein (0.24 mg/g FW) was observed on shoot of 30 g/L ones. In addition, the maximum GPX activity was determined on root of plantlests treated with 30 g/L sucrose (1.52 U/mg protein). In conclude, the concentration of 45 g/L of sucrose can be a good elicitor to increase production of HYOSCYAMINE and SCOPOLAMINE with high medicinal value inD.stramonium which probably due to the fact that sucrose does not only act as a carbon and energy source but also induces signaling pathway of tropane alkaloids synthesis.

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APA: Copy

FATHI REZAEI, P., & RAKEE, E.. (2017). INVESTIGATION OF SUCROSE EFFECT ON TROPANE ALKALOID PRODUCTION AND SEVERAL BIOCHEMICAL PARAMETERS OF DATURA STRAMONIUM UNDER IN VITRO CULTURE CONDITION. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 30(4 ), 558-571. SID. https://sid.ir/paper/363877/en

Vancouver: Copy

FATHI REZAEI P., RAKEE E.. INVESTIGATION OF SUCROSE EFFECT ON TROPANE ALKALOID PRODUCTION AND SEVERAL BIOCHEMICAL PARAMETERS OF DATURA STRAMONIUM UNDER IN VITRO CULTURE CONDITION. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2017;30(4 ):558-571. Available from: https://sid.ir/paper/363877/en

IEEE: Copy

P. FATHI REZAEI, and E. RAKEE, “INVESTIGATION OF SUCROSE EFFECT ON TROPANE ALKALOID PRODUCTION AND SEVERAL BIOCHEMICAL PARAMETERS OF DATURA STRAMONIUM UNDER IN VITRO CULTURE CONDITION,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 30, no. 4 , pp. 558–571, 2017, [Online]. Available: https://sid.ir/paper/363877/en

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