Investigating the anticancer effect of Lippia citriodora leaf alcoholic extract: in suppression of A2780 ovarian cancer cell metastasis via restoration of E-cadherin expression

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AMINI E.; NABIUNI M.; BAHARARA J.; Behzad S.B.; Seyfi D.; Salek F.

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Journal: JOURNAL OF CELL & TISSUE Year:2019 | Volume:10 | Issue:1 Page(s): 24-33

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Title

Investigating the anticancer effect of Lippia citriodora leaf alcoholic extract: in suppression of A2780 ovarian cancer cell metastasis via restoration of E-cadherin expression

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Keywords

Lippia citriodoraQ1

metastasisQ1

epithelial mesenchymal transitionQ1

E-cadherinQ1

ovarian cancerQ1

Abstract

Aim: The purpose of this study is to investigate the pro-apoptotic and anti-metastatic potentials of Lippia citriodora alcoholic leaf extract on A2780 ovarian cancer cells and to evaluate the expression of E-cadherin as one of the most important marker in metastasis. Material and Methods: A2780 ovarian cancer cell line was prepared from Pasteur cell bank and cultured in RPMI1640 medium containing 10% FBS and 1% antibiotic. After cell seeding and treatment with different concentrations of extract (10-400 µ g/ml), the cells viability and pro-apoptotic potential were examined by MTT assay and acridine orange/ propodium iodide, respectively. Caspase3 assay and anti-invasive effect were analyzed by migration assay and assessment of E-cadherin expression, respectively. Then, one way ANOVA test was employed for analysis of quantitative data. Results: Data indicated that L. citriodora alcoholic extract attenuated ovarian cancer cell viability. Acridine orange/ propodium iodide and caspase-3 assays showed that this extract in IC50 concentration (100 µ g/ml) induced apoptosis mainly through caspase dependent pathway in the ovarian cancer cells. Migration assay and RT-PCR exhibited that this extract has anti-invasive capability and by up-regulation of E-cadherin prevents the loss of attachment between cancer cells. Conclusion: The results revealed that L. citriodora alcoholic extract has apoptosis inducing capacity and ability of restoration E-cadherin expression in A2780 cancer cells, which it able to suppress invasive potential of ovarian cancer cells.

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APA: Copy

AMINI, E., NABIUNI, M., BAHARARA, J., Behzad, S.B., Seyfi, D., & Salek, F.. (2019). Investigating the anticancer effect of Lippia citriodora leaf alcoholic extract: in suppression of A2780 ovarian cancer cell metastasis via restoration of E-cadherin expression. JOURNAL OF CELL & TISSUE, 10(1 ), 24-33. SID. https://sid.ir/paper/364454/en

Vancouver: Copy

AMINI E., NABIUNI M., BAHARARA J., Behzad S.B., Seyfi D., Salek F.. Investigating the anticancer effect of Lippia citriodora leaf alcoholic extract: in suppression of A2780 ovarian cancer cell metastasis via restoration of E-cadherin expression. JOURNAL OF CELL & TISSUE[Internet]. 2019;10(1 ):24-33. Available from: https://sid.ir/paper/364454/en

IEEE: Copy

E. AMINI, M. NABIUNI, J. BAHARARA, S.B. Behzad, D. Seyfi, and F. Salek, “Investigating the anticancer effect of Lippia citriodora leaf alcoholic extract: in suppression of A2780 ovarian cancer cell metastasis via restoration of E-cadherin expression,” JOURNAL OF CELL & TISSUE, vol. 10, no. 1 , pp. 24–33, 2019, [Online]. Available: https://sid.ir/paper/364454/en

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