Effect of antimicrobial peptide EC-hepcidin1 on inhibition of Streptococcus iniae in primary cell culture of (Oncorhynchus mykiss)

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GHODSI ZOHREH; KALBASSI MOHAMMAD REZA; MOHEBATI MOBAREZ ASHRAF; FARZANEH PARVANEH

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Journal: AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY Year:2020 | Volume:7 | Issue:4 Page(s): 83-103

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Title

Effect of antimicrobial peptide EC-hepcidin1 on inhibition of Streptococcus iniae in primary cell culture of (Oncorhynchus mykiss)

Author(s)

GHODSI ZOHREH | KALBASSI MOHAMMAD REZA | MOHEBATI MOBAREZ ASHRAF | FARZANEH PARVANEH | Issue Writer Certificate

Keywords

Rainbow Trout Primary CellsQ1

Antimicrobial PeptideQ1

EC-hepcidin1Q1

Streptococcus iniaeQ1

Abstract

Streptococcosis is the most destructive disease in world aquaculture, and many efforts have been made to curb these bacteria. In this study, the ability of artificial polypeptide hepcidin (EC-hepcidin1) was evaluated to control infection caused by Streptococcus iniae in primary cell culture of rainbow trout (Oncorhynchus mykiss). Antibacterial effects of this polypeptide were examined by disk diffusion and minimum inhibitory concentration (MIC) and minimum bactericidal concentration of EChepcidin1 were measured against Streptococcus iniae. The primary cells were produced from fish fin, and the toxicity range of the peptide was determined for the cells. The primary cells of the rainbow trout were infected with Streptococcus iniae and antimicrobial activity of EC-hepcidin1, as well as viability of the cells, were evaluated. Results showed the MIC and MBC were 75 and 150 μ g/mL, respectively. The level of peptide toxicity on fish cells show that EC-hepcidin1 had low toxicity after 48h in the range of 25 to 400 μ g/mL, and the cells had a viability of 60% to 80%. The viability of the cells was 55% after induction of the bacteria (10: MOI), and the survival rate of cells was increased up to 90% after induction of bacteria (10: MOI) with 150 μ g/mL peptide. In conclusion, the EC-hepcidin1 has shown an antibacterial effect on Streptococcus iniae (in vitro) and the peptide has low toxicity to fish cells. It has increased the vitality of cells infected with Streptococcus iniae. Therefore, complementary clinical research on larval stage, as well as broodstock of rainbow trout, could be suggested in the future. In conclusion, the EC-hepcidin1 has been shown an antibacterial effect on Streptococcus and low toxicity to fish cells. It has increased the vitality of cells infected with Streptococcus. Therefore, complementary clinical research on larval stage and broodstock of fish could be suggested.

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APA: Copy

GHODSI, ZOHREH, KALBASSI, MOHAMMAD REZA, MOHEBATI MOBAREZ, ASHRAF, & FARZANEH, PARVANEH. (2020). Effect of antimicrobial peptide EC-hepcidin1 on inhibition of Streptococcus iniae in primary cell culture of (Oncorhynchus mykiss). AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY, 7(4 ), 83-103. SID. https://sid.ir/paper/365717/en

Vancouver: Copy

GHODSI ZOHREH, KALBASSI MOHAMMAD REZA, MOHEBATI MOBAREZ ASHRAF, FARZANEH PARVANEH. Effect of antimicrobial peptide EC-hepcidin1 on inhibition of Streptococcus iniae in primary cell culture of (Oncorhynchus mykiss). AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY[Internet]. 2020;7(4 ):83-103. Available from: https://sid.ir/paper/365717/en

IEEE: Copy

ZOHREH GHODSI, MOHAMMAD REZA KALBASSI, ASHRAF MOHEBATI MOBAREZ, and PARVANEH FARZANEH, “Effect of antimicrobial peptide EC-hepcidin1 on inhibition of Streptococcus iniae in primary cell culture of (Oncorhynchus mykiss),” AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY, vol. 7, no. 4 , pp. 83–103, 2020, [Online]. Available: https://sid.ir/paper/365717/en

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