PRENATAL SEX DETERMINATION USING AMPLIFICATION OF Y-SPECIFIC SEQUENCES IN MATERNAL PLASMA

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PAKRAVAN M.; RASTGAR LARI GH.; ALA F.A.

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Journal Paper

Paper Information

Journal: JOURNAL OF SCIENCE (KHARAZMI UNIVERSITY) Year:2005 | Volume:4 | Issue:3-4 Page(s): 401-409

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Title

PRENATAL SEX DETERMINATION USING AMPLIFICATION OF Y-SPECIFIC SEQUENCES IN MATERNAL PLASMA

Author(s)

PAKRAVAN M. | RASTGAR LARI GH. | ALA F.A. | Issue Writer Certificate

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Abstract

Background: Fetal DNA has been found in maternal plasma. Diagnosis of fetal gender using maternal plasma has been attempted in an effort to develop a new noninvasive method of prenatal diagnosis.Methods: We extracted DNA from plasma of 30 pregnant woman, three men, three non-pregnant women, and performed a sensitive Y-PCR assay to detect circulating male fetal DNA from women bearing male fetuses. Fetus-derived Y sequences were detected using 40 cycles of PCR carried out for each DNA extract. The PCR products were analyzed by 8% acrylamide gel electrophoresis and ethidium bromide staining, and the results were compared after delivery.Findings: Y sequences were detected in three control men and 14 pregnant woman who had a male fetus. Two samples from eleven weeks and 19 weeks women bearing male fetuses gave negative result. None of 8 woman bearing female fetuses and none of the three non-pregnant control women gave a positive Y signal. In one sample from a 12 weeks woman bearing female fetus Y sequences was detected. Interpretation: Conventional PCR analysis of maternal plasma can be used to diagnose fetal gender after 19 weeks. Prenatal sex determination befor 15 weeks needs more sensitive methods such as Quantitative PCR.

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APA: Copy

PAKRAVAN, M., RASTGAR LARI, GH., & ALA, F.A.. (2005). PRENATAL SEX DETERMINATION USING AMPLIFICATION OF Y-SPECIFIC SEQUENCES IN MATERNAL PLASMA. JOURNAL OF SCIENCE (KHARAZMI UNIVERSITY), 4(3-4), 401-409. SID. https://sid.ir/paper/368233/en

Vancouver: Copy

PAKRAVAN M., RASTGAR LARI GH., ALA F.A.. PRENATAL SEX DETERMINATION USING AMPLIFICATION OF Y-SPECIFIC SEQUENCES IN MATERNAL PLASMA. JOURNAL OF SCIENCE (KHARAZMI UNIVERSITY)[Internet]. 2005;4(3-4):401-409. Available from: https://sid.ir/paper/368233/en

IEEE: Copy

M. PAKRAVAN, GH. RASTGAR LARI, and F.A. ALA, “PRENATAL SEX DETERMINATION USING AMPLIFICATION OF Y-SPECIFIC SEQUENCES IN MATERNAL PLASMA,” JOURNAL OF SCIENCE (KHARAZMI UNIVERSITY), vol. 4, no. 3-4, pp. 401–409, 2005, [Online]. Available: https://sid.ir/paper/368233/en

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