Immunohistochemical localization of Ghrelin in sex accessory glands of bull

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Rahmi M.; SHOKROLLAHI B.; AMIRI A.A.

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ISSN: 2588-4824

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Paper Information

Journal: Animal Production Research Year:2019 | Volume:7 | Issue:4 Page(s): 79-88

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Title

Immunohistochemical localization of Ghrelin in sex accessory glands of bull

Author(s)

Rahmi M. | SHOKROLLAHI B. | AMIRI A.A. | Issue Writer Certificate

Keywords

AmpullaQ1

ProstateQ1

Holstein bullQ1

GhrelinQ1

Vesicle seminalQ1

Abstract

Ghrelin is a 28 amino acid peptide hormone that is expressed in the stomach and a range of peripheral tissues. Expression and functional role of Ghrelin in the sex accessory glands of mammals have not been studied yet (except for human). This study was aimed to determine the immunohistochemical position of Ghrelin in Prostate, seminal vesicles and Ampulla tissues of Holstein bulls. Anti-Ghrelin mouse monoclonal antibody was used as primary antibody and donkey polyclonal antibody Anti-IgG Horseradish Peroxidase (HRP) was used as secondary antibody. Samples of sex accessory glands tissues were collected from five Holstein bulls aged two years old, and preserved in 10% buffered formalin for the immunohistochemical test. Then, paraffin blocks and tissue sections were prepared. The immunohistochemical results were positive for the secretory cells of Prostate, seminal vesicles and Ampulla. But the intensity of staining in the Prostate glands was very weak than to the other glands. According to the results of this research, the expression of Ghrelin in some accessory glands of the bull may display its possible role in fertility parameters of sperm and overall fertilization process.

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APA: Copy

Rahmi, M., SHOKROLLAHI, B., & AMIRI, A.A.. (2019). Immunohistochemical localization of Ghrelin in sex accessory glands of bull. ANIMAL PRODUCTION RESEARCH, 7(4 ), 79-88. SID. https://sid.ir/paper/369616/en

Vancouver: Copy

Rahmi M., SHOKROLLAHI B., AMIRI A.A.. Immunohistochemical localization of Ghrelin in sex accessory glands of bull. ANIMAL PRODUCTION RESEARCH[Internet]. 2019;7(4 ):79-88. Available from: https://sid.ir/paper/369616/en

IEEE: Copy

M. Rahmi, B. SHOKROLLAHI, and A.A. AMIRI, “Immunohistochemical localization of Ghrelin in sex accessory glands of bull,” ANIMAL PRODUCTION RESEARCH, vol. 7, no. 4 , pp. 79–88, 2019, [Online]. Available: https://sid.ir/paper/369616/en

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