Assay of NPR1, MLO and BI-1 genes expression in susceptible wheat to powdery mildew after treatment with chitosan

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

KHATAMI M.; AHANGAR L.; TALIEI F.; SABOURI H.; Babaei zad V.A.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Molecular and Cellular Researches Year:2019 | Volume:31 | Issue:4 Page(s): 0-0

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

563

Download:

Cites:

Information Journal Paper

Title

Assay of NPR1, MLO and BI-1 genes expression in susceptible wheat to powdery mildew after treatment with chitosan

Author(s)

Keywords

WheatQ2

ChitosanQ1

Powdery mildewQ1

ResistanceQ2

Abstract

Powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is one of the most important diseases of Wheat that make yield damage annually. In recent years, the use of induction elicitors has been widely welcomed. Therefore, in this research in order to examine the ability of Chitosan as a biotic elicitor, Flat were selected as susceptible cultivar to Powdery mildew, and the leaves were sprayed with Chitosan in the two-leaf stage. Then treated plant together control plants were inoculated with Bgt pathogen. All stages of the were conducted in 2016 in the genetic laboratory in Gonbad-e Kavouse University. Finally, The expression rate of MLO, B-I1 and NPR1 genes was examined in using Real Time PCR technique at 4 time courses and in 3 independent replicates. Results showed that in both Chitosan treated plants and the controls, rate of gene expression were increased after infection for all genes, So that, in the first hours after inoculation, treated plants showed NPR1 expression more rapidly than control. Maximum expression level of this gene was observed at 24 hours after infection. But, in treated plants, the expression rate of BI-1 and MLO were less than the control and they showed minimum expression at 24 hours after infection. These results indicated these genes by decreasing their expression rate in order to increase the level of active oxygen species causes induction of cell death in the plant to inhibition of fungal growth and development in the early stages after infection. The gene expression rate in plants treated with 250 mg/L was more indicative than those treated with Chitosan 500 mg/L. Overall; results showed that Chitosan could induce systemic Resistance in susceptible Wheat against Powdery mildew in low concentration.

Cites

No record.

References

No record.

Cite

APA: Copy

KHATAMI, M., AHANGAR, L., TALIEI, F., SABOURI, H., & Babaei zad, V.A.. (2019). Assay of NPR1, MLO and BI-1 genes expression in susceptible wheat to powdery mildew after treatment with chitosan. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 31(4 ), 0-0. SID. https://sid.ir/paper/370498/en

Vancouver: Copy

KHATAMI M., AHANGAR L., TALIEI F., SABOURI H., Babaei zad V.A.. Assay of NPR1, MLO and BI-1 genes expression in susceptible wheat to powdery mildew after treatment with chitosan. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2019;31(4 ):0-0. Available from: https://sid.ir/paper/370498/en

IEEE: Copy

M. KHATAMI, L. AHANGAR, F. TALIEI, H. SABOURI, and V.A. Babaei zad, “Assay of NPR1, MLO and BI-1 genes expression in susceptible wheat to powdery mildew after treatment with chitosan,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 31, no. 4 , pp. 0–0, 2019, [Online]. Available: https://sid.ir/paper/370498/en

Related Journal Papers