The effect of Punicic Acid (pomegranate seed oil) on metalloproteinase genes (MMP-1, 3) in THP-1 cells stimulated with LPS compared with steroidal and non-steroidal drugs

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Vazirijavid R.; MAGHSOUDI H.; HAJIHOSSEINI R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Molecular and Cellular Researches Year:2019 | Volume:32 | Issue:4 Page(s): 504-515

Download Full-Text

Persian Verion

View:

409

Download:

Cites:

Information Journal Paper

Title

The effect of Punicic Acid (pomegranate seed oil) on metalloproteinase genes (MMP-1, 3) in THP-1 cells stimulated with LPS compared with steroidal and non-steroidal drugs

Author(s)

Vazirijavid R. | MAGHSOUDI H. | HAJIHOSSEINI R. | Issue Writer Certificate

Keywords

Punicic AcidQ1

MMP-1Q1

3Q1

THP-1Q1

OsteoarthritisQ1

Abstract

Osteoarthritis is a common joint disease for which there are currently no diseasemodifying drugs available. Osteoarthritis (OA) affects most of the elderly population, the main features of which are cartilage damage. Degradation of the cartilage extracellular matrix is a central feature of the disease and is widely thought to be mediated by proteinases that degrade structural components of the matrix. The matrix metalloproteinases (MMPs) are a family of human zinc endopeptidases that play significant roles in inflammatory diseases such as Osteoarthritis. In this experiential laboratory study. Punicic Acid of pomegranate seed oil was purchased from Clarodan Kerman Co, . THP-1 cells (Pasteur Institute of Iran) were cultured and administered with concentration of 8 to 100 μ g/ml (in 24h, 48h, and 72h). Cellular toxicity of Punicic Acid against THP-1 was estimated using the MTT assay and to measure the inhibitory effects of PA on Matrix metalloproteinase proteinase activity, ELISA and Western blot, migration and invasion tests were performed. Data were analyzed using T. student and ANOVA. Western blotting and ELISA showed inhibitory effect of Punicic Acid on the expression of MMP-1 but not in MMP-3. Punicic Acid of pomegranate seed oil shows the most cellular toxicity effect at lc50=50 micrograms per milliliters and 72 hours after treatment. According to effect of Punicic Acid on MMP-1 expression, this material can be used as a potential candidate for further studies on the other MMPS and its replacing the chemical drugs.

Cites

No record.

References

No record.

Cite

APA: Copy

Vazirijavid, R., MAGHSOUDI, H., & HAJIHOSSEINI, R.. (2019). The effect of Punicic Acid (pomegranate seed oil) on metalloproteinase genes (MMP-1, 3) in THP-1 cells stimulated with LPS compared with steroidal and non-steroidal drugs. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 32(4 ), 504-515. SID. https://sid.ir/paper/371245/en

Vancouver: Copy

Vazirijavid R., MAGHSOUDI H., HAJIHOSSEINI R.. The effect of Punicic Acid (pomegranate seed oil) on metalloproteinase genes (MMP-1, 3) in THP-1 cells stimulated with LPS compared with steroidal and non-steroidal drugs. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2019;32(4 ):504-515. Available from: https://sid.ir/paper/371245/en

IEEE: Copy

R. Vazirijavid, H. MAGHSOUDI, and R. HAJIHOSSEINI, “The effect of Punicic Acid (pomegranate seed oil) on metalloproteinase genes (MMP-1, 3) in THP-1 cells stimulated with LPS compared with steroidal and non-steroidal drugs,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 32, no. 4 , pp. 504–515, 2019, [Online]. Available: https://sid.ir/paper/371245/en

Related Journal Papers