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Information Journal Paper

Title

Selective Isolation of the Persian Gulf Sponge-associated Actinobacteria and Evaluation of Cytotoxic and Antioxidant activity of Their Metabolites

Pages

  39-48

Abstract

 Sponge-associated actinobacteria are the prolific sources of marine natural products. The aims of the present study were to propose a process for selective isolation of sponge-associated actinobacteria and to find isolates that produce antioxidant and Cytotoxic metabolites. We collected 4 sponge species from the Larak Island, Persian Gulf using scuba diving. The selective isolation process was performed by 4 culture media and 7 physical and chemical treatments. Antioxidant activity of extracted metabolites were evaluated using DPPH radical scavenging activity. Evaluation of cytotoxicity of the extracted metabolites was carry out using brine-shrimp microwell cytotoxicity assay. Out of 114 actinobacterial isolates, 38. 59% was isolated from sponge Dysedia avara. Marine sponge agar medium exhibited maximum efficiency and isolated 44 isolate of actinobacteria. While, most non streptomyces-like isolates were derived by marine zobell agar. Heat treatment isolated 35. 08% of actinobacterial isolates and recovered their most frequency. Approximately, 46 % of the extracted metabolites scavenged more than 90% of the DPPH radicals and their IC50 ranges from 230. 1 to 775. 34 µ g/ml. While, 44% of the extracted metabolites were able to kill more than 90% of the Artemia cells at LC50 range from 236. 3 to 565. 3 µ g/ml. These results represented a selective isolation process for sponge associated-actinobacteria and also could provide an evidence that confirms the active association of the actinobacteria with the Persian Gulf sponges.

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    APA: Copy

    Gozari, Mohsen, TAMADONI JAHROMI, SAEID, POURMOZAFFAR, SAJJAD, BEHZADI, SIAMAK, & HAFEZIEH, MAHMOUD. (2020). Selective Isolation of the Persian Gulf Sponge-associated Actinobacteria and Evaluation of Cytotoxic and Antioxidant activity of Their Metabolites. JOURNAL OF OCEANOGRAPHY, 11(41 ), 39-48. SID. https://sid.ir/paper/377837/en

    Vancouver: Copy

    Gozari Mohsen, TAMADONI JAHROMI SAEID, POURMOZAFFAR SAJJAD, BEHZADI SIAMAK, HAFEZIEH MAHMOUD. Selective Isolation of the Persian Gulf Sponge-associated Actinobacteria and Evaluation of Cytotoxic and Antioxidant activity of Their Metabolites. JOURNAL OF OCEANOGRAPHY[Internet]. 2020;11(41 ):39-48. Available from: https://sid.ir/paper/377837/en

    IEEE: Copy

    Mohsen Gozari, SAEID TAMADONI JAHROMI, SAJJAD POURMOZAFFAR, SIAMAK BEHZADI, and MAHMOUD HAFEZIEH, “Selective Isolation of the Persian Gulf Sponge-associated Actinobacteria and Evaluation of Cytotoxic and Antioxidant activity of Their Metabolites,” JOURNAL OF OCEANOGRAPHY, vol. 11, no. 41 , pp. 39–48, 2020, [Online]. Available: https://sid.ir/paper/377837/en

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