The effect of adding different levels of zinc sulfate on Ghezel ram sperm function during the freeze-thawing process in out of breeding season

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DAGHIGH KIA HOSSEIN; Jafari Sepehr

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Journal: Animal Science Journal Year:2018 | Volume:31 | Issue:120 Page(s): 59-70

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Title

The effect of adding different levels of zinc sulfate on Ghezel ram sperm function during the freeze-thawing process in out of breeding season

Author(s)

DAGHIGH KIA HOSSEIN | Jafari Sepehr | Issue Writer Certificate

Keywords

SpermQ1

Freeze-thawingQ1

Zinc SulfateQ1

RamQ1

Abstract

The purpose of this study was to compare the effect of different levels of Zinc Sulfate on improving the quality of Ram semen during Freeze-thawing process outside of the reproductive season. Semen samples were collected from five Ghezel Ram twice a week using the artificial vagina. Sperm samples were assigned to four treatment groups including: control, 17. 5, 35 and 52. 5 μ m Zinc Sulfate, and then the freezing process was performed. After thawing motility, morphology and malondialdehyde levels of Sperm were studied. Total and progressive motility of Sperm containing the 17. 5 µ mol/ml Zinc Sulfate were significantly higher than control group (P <0. 05). There was a significant difference in Sperm morphology in the group receiving 52. 5 μ mol of Zinc Sulfate compared to the control group, which caused 52. 5% increase in abnormal Sperm (P <0. 05). Adding 17. 5 μ mol of Zinc Sulfate increased unsignificantly the viability of Sperms compared to the control group, but adding 35 and 52. 5 μ mol decreased significantly (P <0. 05). The level of malondialdehyde production in the 35 µ mol was lower than the other treatment groups and the control group and there was a significant difference between the groups 17. 5 and 35 in comparison with the control group (P <0. 05). Therefore, it can be concluded that the use of 17. 5 and 35 µ mol Zinc Sulfate in Ghezel Ram diluent semen improves some of the paRameters of the Sperm after the Freeze-thawing process.

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APA: Copy

DAGHIGH KIA, HOSSEIN, & Jafari, Sepehr. (2018). The effect of adding different levels of zinc sulfate on Ghezel ram sperm function during the freeze-thawing process in out of breeding season. ANIMAL SCIENCES JOURNAL (PAJOUHESH & SAZANDEGI), 31(120 ), 59-70. SID. https://sid.ir/paper/380112/en

Vancouver: Copy

DAGHIGH KIA HOSSEIN, Jafari Sepehr. The effect of adding different levels of zinc sulfate on Ghezel ram sperm function during the freeze-thawing process in out of breeding season. ANIMAL SCIENCES JOURNAL (PAJOUHESH & SAZANDEGI)[Internet]. 2018;31(120 ):59-70. Available from: https://sid.ir/paper/380112/en

IEEE: Copy

HOSSEIN DAGHIGH KIA, and Sepehr Jafari, “The effect of adding different levels of zinc sulfate on Ghezel ram sperm function during the freeze-thawing process in out of breeding season,” ANIMAL SCIENCES JOURNAL (PAJOUHESH & SAZANDEGI), vol. 31, no. 120 , pp. 59–70, 2018, [Online]. Available: https://sid.ir/paper/380112/en

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