Isolation and Identification of Halobacillus sp. SL-7: A moderately halophilic bacterium with potential biological converting L-tyrosine into L-dopa

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ASHENGROPH M.; SAYYADI M.

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Journal: Journal of Molecular and Cellular Researches Year:2020 | Volume:33 | Issue:1 Page(s): 28-41

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Information Journal Paper

Title

Isolation and Identification of Halobacillus sp. SL-7: A moderately halophilic bacterium with potential biological converting L-tyrosine into L-dopa

Author(s)

ASHENGROPH M. | SAYYADI M. | Issue Writer Certificate

Keywords

BioconversionQ1

L-tyrosineQ1

L-dopaQ1

Resting cellQ1

Halobacillus sp.SL-7Q1

Abstract

L-dopa (L-3, 4-dihydroxyphenylalanine) was first discovered in the 1960s as the most effective treatment for Parkinson's disease. In the present research, for the first time, isolation and identification of L-tyrosine-degrading moderately halophilic bacteria and evaluation their ability as biocatalyst for the Bioconversion of L-tyrosine into L-dopa was studied. Using an enrichment technique in tyrosine-containing medium, the Ltyrosine-degrading strain SL-7 with the highest ability to bioconvert L-tyrosine into Ldopa was phenotypically and molecularly characterized and its 16S rDNA sequence was submitted as Halobacillus sp. SL-7. In order to increase the reaction efficiency and prevent the degradation of the metabolites (L-dopa), One-factor-at-a-time optimization method was used to improve the yield of L-dopa in the conversion reaction under Resting cells of Halobacillus sp. SL-7. Based on our results, the optimum Bioconversion conditions for the production of L-dopa can be summarized as follows: initial tyrosine concentration 1. 5 g/l, biomass concentration 7. 5 g/l, copper concentration 0. 03 g/l and peptone at the concentration 1 g/l as cosubstrate. Under these conditions, the maximal L-dopa concentration (0. 75 g/l with a molar yield of 46. 2%) was achieved after a 36 h reaction. This is the first report on the potential of Resting cells of Halobacillus sp. SL-7, A moderately halophilic bacterium with ability biological converting L-tyrosine into Ldopa.

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APA: Copy

ASHENGROPH, M., & SAYYADI, M.. (2020). Isolation and Identification of Halobacillus sp. SL-7: A moderately halophilic bacterium with potential biological converting L-tyrosine into L-dopa. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 33(1 ), 28-41. SID. https://sid.ir/paper/380124/en

Vancouver: Copy

ASHENGROPH M., SAYYADI M.. Isolation and Identification of Halobacillus sp. SL-7: A moderately halophilic bacterium with potential biological converting L-tyrosine into L-dopa. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2020;33(1 ):28-41. Available from: https://sid.ir/paper/380124/en

IEEE: Copy

M. ASHENGROPH, and M. SAYYADI, “Isolation and Identification of Halobacillus sp. SL-7: A moderately halophilic bacterium with potential biological converting L-tyrosine into L-dopa,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 33, no. 1 , pp. 28–41, 2020, [Online]. Available: https://sid.ir/paper/380124/en

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