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Information Journal Paper

Title

Control of osmotic stress tolerance evaluation using Folic acid of Kentucky bluegrass (Poa pratensis cv. Barimpala) in in vitro culture

Pages

  145-159

Abstract

 Considering the high water requirement of turfgrass during growth stages and the limitation of irrigation sources, it is important to find a suitable way to reduce the water requirement of the lawns as ground cover plants used in the Landscape. The purpose of this study was to investigate the effect of 2, 4, 5-T on the induction of Callus from the hypocotyl explant in Kentucky bluegrass, in vitro osmotic stress using Polyethylene glycol and evaluation of the tolerance to stress using folic acid in vitro conditions. Therefore, the research was conducted in four experiments. In the first experiment, the effect of 2, 4, 5-T (0. 5, 1 and 2 mg l-1 ) in combination with two concentrations of 0 and 0. 1 mg l-1 BA on Callus induction in a hypocotyl explant was investigated. In the second experiment, 0,-0. 005,-0. 007,-0. 010, 0. 16 and-0. 02 (bar) concentrations of PEG were used for induction of osmotic stress in calli. In the third and fourth experiments, folic acid was used to suppress osmotic stress induced by PEG in two stages of Callus and regeneratin plants. The results of the first experiment showed that highest percentage of Callus induction at a concentration of 0. 5 mg l-1 of 2, 4, 5-T alone was observed. Based on the results of the second experiment, it was found that increasing the concentration of PEG decreased the growth indices in Callus, so that in the concentration of-0. 016 and-0. 022 bar PEG, the least amount of measured traits of Callus was observed. Also, the results of the third experiment showed that the use of folic acid in MS medium at 0. 5 and 1 mg l-1 concentration reduced Callus growth traits and increased soluble sugar content. In the fourth experiment, the results showed a decrease in the measured traits of regenerated plants in-0. 022 bar PEG. The highest amount of Soluble sugars in regenerated plants was observed in treatment 1 mg l-1 folic acid in combination with 0 bar PEG. In the third and fourth test, folic acid did not affect osmotic stress control.

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    APA: Copy

    Sajadi, Fahimeh Sadat, TAGHIZADEH, MINA, & VALIZADEH KAJI, BABAK. (2020). Control of osmotic stress tolerance evaluation using Folic acid of Kentucky bluegrass (Poa pratensis cv. Barimpala) in in vitro culture. JOURNAL OF PLANT PROCESS AND FUNCTION, 9(35 ), 145-159. SID. https://sid.ir/paper/383781/en

    Vancouver: Copy

    Sajadi Fahimeh Sadat, TAGHIZADEH MINA, VALIZADEH KAJI BABAK. Control of osmotic stress tolerance evaluation using Folic acid of Kentucky bluegrass (Poa pratensis cv. Barimpala) in in vitro culture. JOURNAL OF PLANT PROCESS AND FUNCTION[Internet]. 2020;9(35 ):145-159. Available from: https://sid.ir/paper/383781/en

    IEEE: Copy

    Fahimeh Sadat Sajadi, MINA TAGHIZADEH, and BABAK VALIZADEH KAJI, “Control of osmotic stress tolerance evaluation using Folic acid of Kentucky bluegrass (Poa pratensis cv. Barimpala) in in vitro culture,” JOURNAL OF PLANT PROCESS AND FUNCTION, vol. 9, no. 35 , pp. 145–159, 2020, [Online]. Available: https://sid.ir/paper/383781/en

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