Effect of various concentrations of lipopolysaccharide on in vitro developmental competence of ovine oocyte and metabolites related to the maturation medium

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Ataei Nazari Sara; MOHAMMADI SANGCHESHMEH ABDOLLAH; Bakhtiarizadeh Mohammad Reza; ASSADI ALAMOUTI ALI; Fouladi Nashta Ali

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Journal: Journal of Animal Production Year:2020 | Volume:22 | Issue:3 Page(s): 491-500

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Title

Effect of various concentrations of lipopolysaccharide on in vitro developmental competence of ovine oocyte and metabolites related to the maturation medium

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Keywords

Bacterial cell wallQ1

EweQ1

Infectious diseasesQ1

OocytesQ2

Reproductive functionQ1

Abstract

This study was conducted to evaluate the effect of various concentrations of lipopolysaccharide (LPS) in maritaruta medium on oocyte maturation, oocyte developmental competence and metabolites related to maturation medium including glucose, pyruvate, lactate and glutamine. The experimental treatments were 0 (control), 0. 01, 0. 1, 1 and 10 µ g/ml of LPS in oocyte maturation medium. The cumulus Oocytes complex (COC) were cultured with various concentrations of lipopolysaccharide. After 24 h of oocyte maturation, the medium was collected and the rates of oocyte maturation, cleaved oocyte and Oocytes reached to blastocyst stage were analyzed. Oocyte maturation rate was lowest in the treatment with 10 µ g/ml of LPS (P<0. 05). Among the measured metabolites, only glucose concentration was linearly decreased in response to increasing levels of LPS in the maturation medium (P<0. 05) as treatment with 10 µ g/ml of LPS had lower glucose concentration comparing to other treatments. The percentage of oocyte cleavage was significantly lower in treatment with highest level of LPS compared to other treatments (P<0. 05). In addition, the treatment with 1 and 10 µ g/ml of LPS significantly reduced blastocyst rate compared to control group (P<0. 05). According to results of this study, lipopolysaccharide could have detrimental effects on oocyte development and these influences seems to be mediated through pathways related to energy metabolism. Acquiring managerial approaches to control LPS enhancing agents during reproductive season could prevent animal's reproductive failure.

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APA: Copy

Ataei Nazari, Sara, MOHAMMADI SANGCHESHMEH, ABDOLLAH, Bakhtiarizadeh, Mohammad Reza, ASSADI ALAMOUTI, ALI, & Fouladi Nashta, Ali. (2020). Effect of various concentrations of lipopolysaccharide on in vitro developmental competence of ovine oocyte and metabolites related to the maturation medium. JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE), 22(3 ), 491-500. SID. https://sid.ir/paper/386389/en

Vancouver: Copy

Ataei Nazari Sara, MOHAMMADI SANGCHESHMEH ABDOLLAH, Bakhtiarizadeh Mohammad Reza, ASSADI ALAMOUTI ALI, Fouladi Nashta Ali. Effect of various concentrations of lipopolysaccharide on in vitro developmental competence of ovine oocyte and metabolites related to the maturation medium. JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE)[Internet]. 2020;22(3 ):491-500. Available from: https://sid.ir/paper/386389/en

IEEE: Copy

Sara Ataei Nazari, ABDOLLAH MOHAMMADI SANGCHESHMEH, Mohammad Reza Bakhtiarizadeh, ALI ASSADI ALAMOUTI, and Ali Fouladi Nashta, “Effect of various concentrations of lipopolysaccharide on in vitro developmental competence of ovine oocyte and metabolites related to the maturation medium,” JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE), vol. 22, no. 3 , pp. 491–500, 2020, [Online]. Available: https://sid.ir/paper/386389/en

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